| Objective: To investigate and compare the expression of serum interleukin 12(IL-12) and interferon-gamma (INF r) level in patients with acute leukemia and healthy donors and to observe the effect of chemotherapy on the expression of IL-12 INF r and LDH in those AL patients.Methods: Fifteen untreated or relapsed patients with acute leukemia (AL), fifteen AL patients obtained complete remission (CR) and sixteen healthy volunteers were entered this study. One to two days before and after chemotherapy 2ml venous fasting-blood sample was collected from those AL patients or from healthy donors in the morning. Then the sera were routinely separated and preserved in - 80℃ refrigerator. The serum concentration of IL-12 and INF y were indirectly tested by Elisa method and the serum LDH concentration was tested by clinical chemistry analyzer. Heart, liver, kidney dysfuction and infective disease were ruled out in all of those patients studied. AL group with 15 untreated or relapsed patients included male 10 cases, female 5 cases, age 8-81 years, average 39.96 years. According to FAB classify: M13 cases M22 cases M33 cases M42 cases L24 cases L31 case. CR group with 15 AL patients included 5 cases obtained CR prior to this project begin and 10 out of 15 untreated or relapsed patients. CR group contained male 9 cases, female 6 cases, age9-78 years, average 41.75 years, according to FAB classify: M13 cases M23 cases M33 cases M42 cases L23 cases L31 case. Finally, the levels of IL-12 ,INF Y and LDH activity of the normal control group the CR group and the untreated or relapsed AL group were compared and analyzed.Results: The level of IL-12, INF r and LDH activity of the untreated or relapsed AL group were 84.93±42.21pg/mh 2.149 ± 0.819 pg/ml and 573.40 ± 202.73u/l respectively. After chemotherapy, they were 151.91 ± 107.07pg/mK 1.199 ± 0.510 pg/ml and 308.80 ± 156.01 u/1. The results showed that the level of IL-12 increased and the levels of INF r LDH decreased significantly. In the CR group, the levels of IL-12 INF r and LDH were 118.86 ± 36.19pg/ml> 5.991±3.275pg/ml and 125.26 ± 48.71 u/1, and the chemotherapy could downregualte them to 54.69 ± 23.09pg/ml, 3.389± 1.940pg/ml and 111.20 ± 42.13 u/1 respectively suggesting that the levels of IL-12, INF r decreased apparently. The levels of IL-12 and INF y in normal group were 96.83±28.95 pg/ml and 1.047± 0.355pg/ml. These results showed that the level of IL-12 was same between the normal group and CR AL group and very low in the untreated or relapsed group. The level of INF y in the CR group was higher than that of the untreated or relapsed group. The significant relationship was observed between normal group and the CR AL group, but not in the untreated or relapsed group before and after chemotherapy and in the CR AL group being chemotherapy.Conclusions: The expressions of EL-12 and INF y are Extremely low in the untreated or relapsed AL group and high in the CR group suggesting that the inhibition of entire immunologic system in vivo by the numberous leukemic cells. On the contrary,the high concentration of IL-12 and INF y in CR patients with acute leukemia can be reduced gradually by chemotherapy impling that the extensive chemotherapy could exert its negative effort on the immunity of leukemic patients. The level of IL-12 in the untreated or relapsed patients increase meanwhile thelevel of LDH as a load of leukemic cells decrease after chemotherapy because of many leukemic cells killed. From this point chemotherapy can also increase immunity of leukemia patients indirectly. |
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