Expression And Effect Of Transforming Growth Factor-βⅠ And Its Receptors In Endometrium Of Implantation Window

Objective (1) To profile the protein expression of TGF 1 and its receptors in cycling and early pregnant endometrium, and to evaluate the biological effects of TGF 1 on uterine endometrium, the window of implantation and its relation with unexplained infertility.(2) To investgate the protein expression of TGF 1 and its receptors in endometrium of hyperandrogenism, and furtherly clarify the effects of TGF 1 on endometrial secretory function and the effect of hyperandrogen on endometrium.Methods (1) Expression of TGF 1 and its receptors are detected cases proliferative phase of in 25 cases, secretory phase of 29 cases, early pregnant endometrium of 12 cases and unexplained infertile implantation endometrium of 12 cases, respectively, by immunohistochemical technique.(2) In the same time, histochemistry techniques are also used for detecting secretory endometrial protein expression of TGF 1 and its receptors in high androgen levels of 25 cases, and that of in 30 cases control, respectively.Results (1) Compared with early and middle proliferative endometrium, immunostaining of TGF 1 and its receptors increase markedly in late proliferativeendometrium in epithelial cells (P<0.05). TGF 3 1 and its receptors are expressed abundantly in mid-luteal phase and early deciduas, and it was different singificantly from that of early secretory phase ( PO.01) . (2) Serum high testosterone and androstenedion level can cause poor morphologic secretory endometrium. Compared with nomal woman, there are markedly statistical differences on low expression of TGF P 1 and TGF P RII in hyperandrogen endometrial stromal cells duing secretory phase. (P<0. 05)Conclusion (1) TGF P 1 plays an important role on the regulation of endometrial cell proliferation and differentiation, and the maintain early pregnancy. Low TGF P 1 in imbed endometrium can cause infertility. (2) High levels of androgen lead a detrimentrial effect on endometrial function. It is a primary factor in infertile and recurrent miscarriage woman of hyperandrogenism.