Affection Of Obstruction Jaundice In The Rats Under Hepatectomy

Objective: To investigate the affection of obstructive jaundice on the Wistar rats with hepatectomy and to detect the changes in common bile duct diameter, pressure and serum bilirubin after the obstruction. Methods: One hundred and thirty Wistar rats weighing 250-300g were adopted in this study. The common bile duct of the rat was ligated to establish the animal. The diameter of common bile duct diameter, pressure and serum bilirubin in ten rats were measured on the 0, 1st, 3rd, 5th, 7th, 14th, 21st day respectively after the ligation. Then, the others (120 rats) underwent 30%, 70% Hepatic resection + Choledochojejunostomy on the 1st, 3rd, 5th, 7th day after the ligation. The level of TPR, ALB, AJG, TBIL, DBIL, ALT in serum and liver ALT, Na+K+-ATPase were measured to value the result of the operation. Results: (1) The diameter of common bile duct on the 0, 1st, 3rd, 5th, 7th, 14th, 21st day after the ligation was l.l 0.2mm, 2.3 0.2mm, 3.2 0.2mm, 5.4 0.3mm, 6.1 0.1mm, 6.2 0.3mm and 6.2 0.2mm respectively. The biggest diameter could be found on the 7th day after the ligation, then it maintained. (2) The pressure of common bile duct on the 0, 1st, 3rd, 5th, 7th, 14th, 21st day after the ligation was 4.5 0.4cmH2O, 6.2 0.5cmH2O, 10.5 0.7cmH2O, 11.3 0.3cmH2O, 15.1 0.2cmH2O, 15.2 0.3cmH2O and 15.2 0.2cm H2O respectively. The highest pressure could be found on the 7th day after the ligation, then it maintained. (3) The level of bilirubin on the 0, 1st, 3rd, 5th, 7th, 14th, 21st day after the ligation was 3.46 1.13umol/l, 65.5 7.8umol/l, 106.7 11.2umol/l, 98.7 4.9umol/l, 11.8 2.5umol/l, 6.9 1.58umol/l and 1.2 0.32umol/l respectively. The highest lever could be found on the 3rd day and then descended. The curve of serum bilirubin metabolism in the Wistar rats was different with human beings. (4) The survival rate after the operation on the group 1, 2, 3, 4, 5, 6, 7, 8 was 20%, 20%,100%, 50%, 30%, 20%, 0 and 0 respectively. The very short time between the two operations, but not the liver dysfunction, might be responsible for the higher mortality in group 1 and 2. Conclusions: (1) 30% Hepatic resection + Choledochojejunostomy is performed on the 3rd day after the ligation of common bile duct is safe with 100% survival rate (P<0.05). However, 70% Hepatic resection + Choledochojejunostomy is dangerous in this time with 50% survival rate only. (2) 30%, 70% Hepatic resection + Choledochojejunostomy is performed on the 5th, 7th day after the ligation is very dangerous with the zero survival rate after the operation. (3) The affection of obstructive jaundice by the ligation of common bile duct in the Wistar rats to undergo Hepatectomy is remarkable.