| ObjectiveThe invasive growth, metastasis and prognosis of malignant neoplasms are dependent to the neovascularization. The neoangiogenesis of malignancies is a complex course, which is the net outcome of the regulatory imbalance to the positive regulators and negative ones. The malignant cells can self-secrete multiple positive regulators. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are two of the most important positive regulators. VEGF is the only diffusible endothelial cell-specific mitogen that induces endo-thelial-cell proliferation and increases vascular permeability, so it is also called vascular permeability factor ( VPF). VEGF is a central angiogenesis-stimulator. The biological functions of bFGF include: (1) to induce the expressions of pro-teinases and urokinase plasminogen activators receptors ( uPAR) , and to regulate to produce integrines. (2) to induce the pre-vascularization changes, such as the formation of vessel-like structures, production of collagen, and et al. ( 3) to stimulate the secretion of VEGF, and to affect the angiogenesis together. (4) to enhance the anti-apoptosis ability of the vascular endothelial cells.It has become increasingly certain that angiogenesis, or new blood vessel formation, plays a predominant role in the malignant growth, invasion and metastasis phenotype. The Antiangiogenic therapy can cut the pathway of neovascularization of malignancies, and increase the opportunities of other radical treatments. The gallbladder carcinoma has the poorest prognosis, so the antiangio-genic therapy seems to be of the great significance. This study aims to illustrate the relationships between the expressions of VEGF, bFGF and microvessel counts in gallbladder carcinomas. We hope the result of this research can pro-vide an evidence for the clinic and the future studies about the antiangiogenic therapies targeting the angiogenic factors, such as VEGF, bFGF and et al.Materials and MethodsForty-nine cases of gallbladder carcinomas were randomly selected and diagnosed histologically. All the patients were treated surgically in the first and second hospitals of China Medical University. The stains of VEGF, bFGF and MV were completed by the Streptavidin-Peroxidase complex immunohistochemi-cal method in routine paraffin-embedded sections of 49 cases of gallbladder carcinomas. MVCs were assessed according to the method of Weidner et al. The expressions of VEGF and bFGF scored semi-quantitatively with corresponding rates of positive cells and stain intensity.ResultsThe microvessels in malignant tissues were heterogeneously distributed. These highly neovascularized areas distributed within the tumor and dominated a-round the tumor margins. The VEGF and bFGF were stained in cytoplasms of gallbladder carcinoma and endothelial cells. MVC in 49 cases with gallbladder carcinomas was (35 12)/HP. The positive rates of VEGF and bFGF in 49 cases with gallbladder carcinomas were respectively 63. 3% (31/49) and 53. 1% (26/49).The expressions of VEGF and bFGF were significantly lower in cases of Ne-vin staging Si-S3 and without metastasis than that of Nevin staging S4-S5 and with metastasis( P < 0.05 ). The expressions of VEGF and bFGF were not correlated with tumor differentiation( P > 0.05 ). The average of MVCs was markedly higher in cases of Nevin stages S4-S5 and with lymph nodes metastasis than in those of Nevin stages SI-S3 and without lymph nodes metastasis ( P <0.01, P < 0.01). The average of MVCs in moderately or poorly differentiated group was higher than that in well-differentiated group and papillary adenocarcinoma group (P <0. 05 ). The average of MVCs in VEGF ( + ) cases was (37 11 )/HP,which was significantly higher than that in VEGF (-) cases (30 12/HP, t = 2.06, P<0.05). The average of MVCs in bFGF ( + ) cases was (40 10)/ HP, which was significantly higher than that in bFGF (-) cases (28 11/HP, t=4.04, P<0.01).ConclusionMVC was correlated to Nevin staging, lymph nodes metastasis and tumor different... |
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