Effects Of Cordyceps Sinensis On Apoptosis And BFGF MRNA Expression In LLCPK Cells Induced By Gentamincin

Objective To investigate the theraperatic effects of Cordyceps Sinensis in Gentamincin-induced cytotoxicity, apoptosis and bFGF gene expression in LLCPK.Methods Model group of LLCPK cells was stimulated with Gentamincin, and at same time added Cordyceps Sinensis in the theraperatic group. The number of cell survival was measured by MTT method and NAG level in culture supernatant was detected by color reaction. Flow cytometry was used to detect the Annexin V upon the cytomembrane. The bFGF mRNA expression was quantitated by RT-PCR assay.Results In this study, after treatment with Gentamincin, the number of cell survival was markly decreased, and NAG level was markly increased. Results presented that Gentamincin could induce the apoptosis and high expression of bFGF mRNA in LLCPK cells. And following incubation with Cordyceps Sinensis, the number of cell survival was significant increased, the level NAG and apoptosis and bFGF mRNA expression was decreased obviously . Cordyceps Sinensis could decrease the cell damage reduced by Gentamincin and inhibite the apoptosis and bFGF mRNA expression in LLCPK cells.Conclusion Cordyceps Sinensis can Proteel the cell damage,olecrease the cell's apoptosis and inhibite the bFGF mRNA expression in LLCPK cells induced by Gentamincin,that could beone of the mathanisms of its cutifibrosis.