Study On A Detection Of Insertion/Deletion Polymorphism Of Angiotensin Ⅰ Converting Enzyme Gene In Pregnacy Induced Hypertension

Objective To detect the Insertion/Deletion Polymorphism of Angiotensin Converting Enzyme Gene with Polymorphism chain reaction (PCR) and to study the relationship between ACE gene Insertion/Deletion Polymorphism and Pregnacy Induced Hypertension(PIH).Methold 　 100 patients with Pregnacy Induced Hypertension and 100 normal pregnant womem as controls were investigated. Polymorphism chain reaction for detection of ACE gene Polymorphism was performed to show the Insertion/Deletion Polymorphism in intron 16 of ACE gene. A 490bp(I) and 190bp(D) PCR products were identified, corresponding to the PCR amplification of the allele with or without the insertion..Results The subjects were classified, according to the presence or absence of a 287bp insertion in intron 16 of the ACE gene, as insertion homozygote (II), deletion homozygote (DD),　or heterozygotesfor insertion/deletion(I/D). The percentage of the ACE DD , ID ,II genotype were respectively as bellows: 0.14(14/100), 0.43(43/100), 0.43(43/100) in the PIH group and 0.14(14/100), 0.46 (46/100), 0.40(40/100) in the control group. The frequency of allele gene D type, allele gene I type were 0.355 (71/200), 0.645(129/200) in the PIH and 0.37(74/200), 0.63(126/200) in the normal pregnant women. The percentage of the ACE DD genotype , ID genotype and II genotype in the PIH group were not significantly different as compared with the percentage in the control group respectively. The frequency of allele gene D type and allele gene I type in the PIH group were not significantly higher as compared that in the control group respectively.Conclusions ACE gene polymorphism included: insertion homozygote (II), deletion homozygote (DD) and insertion/ deletion heterozygote (I/D).The percentage of DD,ID or II show no significant differences between PIH and normal tensive pregnant women. The frequency of allele gene D type and allele gene I type in the PIH group were not significantly higher as compared that in the control group respectively. It is suggested that the Insertion/Deletion Polymorphism in intron 16 of ACE gene may have no association with the pathogenesis of PIH.