The Study Of Tumour Infiltrating Lymphocytes On Malignant Pleural Effusion Of Lung Cancer About Its Proliferative Index, Epitope Analysis, Cytotoxic Activity And Clinical Application

Objective: To study the specific property of the biological activity about tumour infiltrating lymphocytes (TIL) on malignant pleural effusion of lung cancer. To explore the therapeutic effect, side effects and the immunity function of TIL on auto pleural effusion in patients with lung cancer.Methods: To use the interleukin-2(IL-2) to guide and culture the tumour infiltrating lymphocytes on malignant pleural effusion, to count TILs proliferative index according to the routine way, analyze TILs epitope to use the flow cytometry (FACS) and test the cytotoxic activity by the MTT methods. TIL cultured and amplified product of cancer patient's own malignant pleural effusion was used for treatment by intrapleural injection once every other day for four times at least.Results: After 21 days ,according to apply IL-2 to guide and culture TILs , the patients of the TILs increment number over 1000 times were 77%. CD3+ was no difference outstanding(P>0.05) , but CD4\ CD8+ were significantly higher than other days (P<0.01) , then CD4+/CD8+ was a high level of 3.53±0.82 , and TILs were provided with higher cytotoxic activity. In 22 cases treated, lymphocytes in pleural effusion increased, whiletumour cells gradually decreased, and pleural effusion gradually absorbed. After 1 month, 19 cases were effective(86%). Adverse effects were mild. The immunity function of all patients was improved after infusion.Conclusions: It will be acquired the reliable curative effection on malignant pleural effusion, this TILs had gained higher biological activity when the TILs on malignant pleural effusion were cultured in the 21st day.TILs of auto pleural effusion might be effective for malignant pleural effusion.