| In 21st century, much emphasis is put on the quality of life of people, especially on the visual function, but some ocular diseases, including traumatic optic neuropathy, always result in primary or secondary loss of vision. Theoretically, in order to solve the problem in the end, it is the only way to make the injuried optic nerves regenerate completely. Therefore, it is great important for clinical and laboratory works to study the regeneration of the optic nerves. Now it has been proved that the optic nerves of mammal animal have no capacity to regenerate through the injuried site under the normal physiological conditions. However, the optic nerves of some low vertebrates and the peripheral nerves of mammal animals can regenerate and restore the visual function under the situation of injury. Recently, Leon and Fischer found that the injuried lens of rats which result in cataract can greatly promote the regeneration of injuried optic nerves. But so far, we still don't know why injuried lens has so surprised effects on the regeneration of optic nerves.Objective Our research is to study the effects of lens epithelium cells on the regeneration of the optic nerves during the period of lens injury.Method The laboratory animals are Wistar rats. First, we cut the optic nerves of rats completely 0.5 mm behind the eyeball and suture it with the right position. Meanwhile, we also transplant the uninjuried or preinjuried lens epithelium cells between the proximal and distal stumps of the sectioned optic nerves. And through the antegrade labelling method of RITC (rhodmine-B-isothiocyanate) which is injected into vitreous cavity, we can observed the situation of the regeneration of optic nerves. Using this kindof animal model we can study and analyse the function of lens epithelium cells which is one of the important elements of lens during axons regeneration of optic nerves. Results1. Different groups have the similar process of degeneration after injury in their outer shape and average diatmeter of optic nerves, but lens injuried group and preinjuried transplantation group are one week earlier than simple injuried group and uninjuried transplantation group.2. There appeared clustered glial scar with dense astrocytes between the distal and proximal stumps of optic nerves in unregenerating group, but no glial scar in regenerating group, and with plenty of macrophages infiltrating into the injuried site of optic nerves.3. It has a certain incidence of regenerating after injury of optic nerves under certain condition. There are no difference between simple injuried group and uninjuried transplantation group ( x2=3.333, P>0.05) , lens injuried group and preinjuried group( x2=2.581, P>0.05) statistically. But there are significant difference statistically between simple injuried group and lens injuried group( x2=39.23l, P<0.01), simple injuried group and preinjuried transplantation group( x2=26.250, P<0.0 1), uninjuried transplantation group and lens injuried group( x2=39.200, P<0.0 1 ) ., uninjuried transplantation group and preinjuried transplantation group( x2=24.757, P<0.0 1).4. Injuried optic nerves can regenerate a long distance throughout the visual pathway once the regenerating axons penetrating the injuried site of optic nerves(r=0.536).5. The injuried lens which caused cataract can enhance the number of living retinal peripheral RGCs(F=1789.0, P<0.0 1).Conclusions1. It is a good method of antegrade labelling optic nerves axons using RITC as a fluorescent dye.2. Injuried lens which caused cataract greatly promote the regeneration of axons of injuried optic nerves.3. The change of injuried lens epithelium cells play a important role in the process of injuried lens promoting the regenerating of optic nerves.4. The injuried lens which caused cataract can enhance the number of living retinal peripheral RGCs.5. Injuried lens inhibit the formation of glial scar as a physical barrier between two stumps of the optic nerves.6. The c... |
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