A Study On The Relationship Between Respiratory Tract Viruses In Renal Tissue And Urine From Children With Steroid Responsive Simple Nephritic Syndrome

OBJECTIVE: To explore whether respiratory tract viruses infection was involved in the pathogenesis of Steroid responsive simple nephritic syndrome (SRSNS).METHODS: (1)The urine of 28 children with SRSNS (active stage group )underwent the examination of respiratory tract viruses (RT-PCR and APAAP method) when they were hospitalized from Decenber 2001 to April 2003. Among them, 10 were selected as Remission stage group when their proteinuria was negative for at lest four weeks . Moreover, 18 children with nephritic nephrosis ,15 secondary glomerulopathy, 16 with respiratory infection in the same season and 15 healthy children were chosen as the control and were subjected to the examination of respiratory tract viruses by by the same method.(2)RT-PCR amplification production is sequencinged after purification; (3)By with the method of immunohistochemistry ,the antigen of viruses were assayed in renal tissue.RESULTS: The positive rate of viruses in the active stage group of SSSNS children increased (P < 0.05), compared with that in the remission group andthe other control groups. There are no relationship between the positive rate of respiratory tract viruses gene and antigen in the urine of active stage group and the degree of response to predione. we had discovered that the antigen of respiratory track virus detected in the tissue from renal biopsy keeps the same with RT-PCR, APAAP result in urine .CONCLUSION: respiratory tract viruses perhaps plays an important role in the triggering of this disease.