| It is hypothesized that human adipose tissue is an abundant reservoir of adult stem cells, which have potentent to differentiate into many mature cell lineages varying from adipogenic precursors, osteogenic precursors, chondrogenic precursors to myogenic precursors.This research includes three main parts:(1) Isolation and cultivation: Human subcutaneous adipose tissue was acquired by liposuction. The tissue was then digested with one volume of collagenase type I and cultured with BGJb medium.(2) Mineralizing induction: Human adipose tissue-derived stromal cells were cultured and differentiated into osteoblasts in midium of DMEM/F12, supplemented with L-ascorbate, β-glycerophosphate, dexamethasone, 1,25-dihydroxy vitamin D3.(3) Histochemical stains: HE staining, Von Kossa's histochemical staining and alkaline phosphatase assay were employed to identify the osteogenic phenotypes of human adipose tissue-derived stromal cells after osteogenic induction.The results of this research showed: Human adipose tissue -derived stromal cells were able to differentiate into osteoblasts under condition of mineralizing induction. The alkaline phosphatase activity and extracellular calcified nodes were positive among these cells.It seems that human adipose tissue is of adult stem cells, which with potential to differentiate into osteoblasts in vitro. This study may provide some practical methods for engineering bone in future. |
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