Expression Of Nm23, MMP-2, TIMP-2 In Breat Tumor And The Realation Ship In Invasion And Metastasis

The carcinoma of the breast is one of the most popular malignant tumors in women. In recent years, the incidence of it in our country is increased obviously and occupies the first place in malignant tumors. Although the therapy of this cancer is fairly effective, its incidence and mortality rate are very high and its viability rate is still dissatisfied with people, it threatens to women's health heavily. Now, it's formed that tumors invasion and metastasis is the main reason which results in the patients' death. In recent years, study proved that nm23 is an anti-metastalic oncogene, which participates in the invasive and metastatic course and the developmental course. It's low expression show low matastasis. Participate in the invasion and metastastatic course of the tumor, matrix metallo-proteinase(MMPs) and it's inhibitor. Tissue inhibitor of the metalloproteinases(TIMPs),are very important for the degradation of the ECM, and connected with invasion and metastasis.In order to approach the reason and developmental regulation of the breast cancer, and expression of nm23, MMP-2 , TIMP-2 in breat tumor and the realation ship in invasion and metastasis, this study applied theimmunohistochemistry technique SP method, detected the expression of nm-23, MMP-2 and TIMP-2 in the 20 breast fibroaderoma, 24 breast ductal carcinoma in situ and 58 breast invasive ductal carcinoma specimens,to provide theoretical and experimental basis for the progrosis and therapy of the patients. Materials and MethodsThe studying specimens were as follows: 1) all the patients were women. 2) the cases had complete clinical data . 3)and no anti-cancer therapy before surgery. We collected 20 breast fibroaderoma, 24 breast ductal carcinoma in situ and 58 breast invasive ductal carcinoma specimens, (The invasive ductal carcinoma patients had been performed by modified radical mastectomy, we divided them into 2 groups: Group 1 included 26 specimens having no the same directional lymph-node metastasis. Group 2 included 32 specimens having lymph-node metastasis. ) All the specimens came from our hospital in the year from 2000 to 2002. TheTsurgical specimens must be 10%formalin fixed , dehy-drated and paraffin embedded, the paraffin mustbe filed. This study applied the immunohistochemistry technique SP method, detected the expression of nm-23, MMP-2 and TIMP-2 in the 102 breast tumors Results. Result:The positive immunoreaction of these three on coproteins-nm23, MMP-2 and TIMP-2-appeared brown grain distribution. Nm23 oncoprotein was localized in the cytoplasm of the tumor cells, MMP-2 and TIMP-2 oncoproteins were localized in the cytoplasma of the tumor and stromal cells.The statistial analysis use x2 check.l.In the fibroadenoma, the ductal carcinoma in situ and the invasive ductual carcinoma of the breast 4 groups , the positive immunostaining rate of nm23 were 80.00%(17/20) , 87.50%(21/24) , 65.38%(17/26) , 40.63%( 13/32) respectively. There was significant difference among 4 groups (p<0.05).2. In the fibroadenoma, the ductal carcinoma in situ and the invasive ductual carcinoma of the breast 4 groups, the positive immunostaining rate of MMP-2 were 10.00%(2/20) , 54.17%(13/24) , 34.62%(9/26) , 53.12%( 17/32). there was significant difference among 4 groups (p<0.05). 3.In the fibroadenoma, the ductal carcinoma in situ and the invasive ductual carcinoma of the breast 4 groups, the positive immunostaining rate of TIMP-2 were 95.00%(19/20)> 91.67%(22/24), 61.54%(16/26), 50.00%( 16/32) respectively, there was significant difference among 4 groups (p<0.05).4. The expression of nm-23 had significant difference between the ductual carcinoma in situ group and the invasive ductual carcinoma, comparing the lymph-node metastasis negative group with the lymph-node metastasis positive group, the expression of nm-23 had significant difference (p<0.05). These results showed with the decline of nm-23 positive expression rate, the ability of the tumor's geneses, invasion and metastasis...