An Investigation On The Hemorheological Properties Of The Patients Suffering From Chronic Pulmoanry Heart Disease

Objectives:Chronic pulmonary heart disease seriously threaten human's health. So it is important topic to prevent and treat pulmonary heart disease. The study on hemorheology is beneficial to clarify the pathophysiology of chronic pulmonary heart diseases and formulate effective prevention and treatment measure. The study firstly addresses the change of hemorheology of the acute phase patients suffering from chronic pulmonary heart disease in macroscopic, then the study focuses the mechanism that erythrocyte deformability decrease and intervention study of Shuxuetong using micropipette aspiration technique. The text will try hard to hint that it is one of important treatment measure to improve microcirculation disorder of the acute phase patients suffering from chronic pulmonary heart disease both macro- and microscopically.Methods: Using fully automatic viscometer(FASCO-94A), the authors investigated the macroscopic hemorheology of the acute phase patientssuffering from chronic pulmonary heart disease, and using the micropipette aspiration technique, the authors then investigated the membrane elastic modulus, viscous coefficient, surface /volume ratio of the erythrocytes of the patients. And Finally, the effect of Shuxuetong was investigated microscopically. Results:The blood of acute phase patients suffering from chronic pulmonary heart disease was in hyperviscosity state. The blood viscosity, plasm viscosity, hematocrit, aggregation indexes, erythrocyte sedimentation were higher as compared to healthy controls, while erythrocyte deformability index decreased. The acute phase patients suffering from chronic pulmonary heart disease was in contrast to normal human, the erythrocyte surface/volume ratio decreased, while the spherical indexes, membrane elastic moduli and viscous coefficients of the erythrocytes increased. Upon treatment with Shuxuetong in a concentration range of 2.5ug/ml, 5ug/ml, 10ug/ml, 20ug/ml, the surface/volume ratio of erythrocytes were higher as compared to the surface/volume ratio of erythrocytes of the patients, this is most significant at 2.5ug/ml(1.527±0.051 VS 1.340±0.045,P〈0.001〉; The spherical indexes were lower than those of patients, this decrease is also most significant at an agent concentration of 2.5ug/ml(0.787±0.018 VS 0.858±0.012 , P<0.001). Treatment with Shuxuetong in a concentration of 2.5ug/ml, led to a decrease in themembrane elastic moduli (3.122±0.481 VS 6.970±0.672, P<0.001)and an increase in viscous coefficients of the erythrocytes(1.182±0.701 VS 0.936±0.242, P<0.05). The membrane elastic moduli(5ug/ml:2.881±0.390 VS 6.970±0.672, P<0.001; 10ug/ml: 4.855±1.349 VS 6.970±0.672, P<0.001; 20ug/ml: 4.328±0.403 VS 6.970±0.672, P<0.001) and viscous coefficients(5ug/ml:0.450±0.194 VS 0.936±0.242, P<0.001; 10ug/ml: 0.628±0.128 VS 0.936±0.242, P<0.05; 20ug/ml: 0.288±0.061 VS 0.936±0.242, P<0.001) decreased with Shuxuetong in concentrations of 5ug/ml or higher.Conclusion: The decrease of erythrocyte deformability relates to the decrease of the surface/volume ratio, the increase of spherical index, the increase of red cell membrane elastic modulus,viscous coefficient. Shuxuetong can improve erythrocyte rheological properties by decreasing spherical indexes, membrane elastic moduli and viscous coefficients. The study offers experimental evidences that the comprehensive treatment of pulmonary heart disease should involve the drug or measure to improve the erythrocyte deformability...