An Experimental Investigation Of The Function Of Sodium Hyaluronate On Corneas Preservation In Vitrio

Objective:To study the action of SH in short-term and midterm cornea preservation and assess how SH affects the structures and functions of corneal endothelial cell, for improving the cornea preservation ways and effect.Methods: (1)Short-term preservation :fresh eyeballs of New Zealand's white rabbits were divided into three groups: A. injected 1％SH 0.25ml into anterior chambers as soon as empty aqueous humor one by one .B. didn't deal with anterior chambers. C. inject disinfected air 0.25ml into anterior chambers as soon as empty aqueous humor. All eyeballs of these groups were preserved in containers at 4℃.Respectively at 24 .48 .72 . and 96h operation of after preservation——observe corneal appearances besides slit-lamp microscope ,biomicrescope and trypan blue-alizarin red staining, inorder to detect the vitality of corneal endothelial cells. (2)Midterm preservation: make up reformed preservation liquids-MMK(referred to M-K prescription) and added SH into it to produce the preservative Ⅰ,Ⅱ,Ⅲ. Consistency of SH in these groups: Ⅰ0.05％,Ⅱ0.03％, Ⅲ 0.01％.Pure MMK was the contrasted. Preserve rabbit's corneas at 4℃.respectively at 3 .5 .7 .10days (within the preservation period), after risen to (30~34℃) and continued for 2h,detected vitality of corneal endothelial cells with trypan blue-alizarin red staining, detected vitality of SDH and G-6-P enzyme with tissue-chemical staining, observed ultrastructures of corneal endothelium in TEM and SEM.Results : (1)hort-term preservation: 24h: A corneal surfaces were all smooth and transparent without any edema. No ruga in Descemet's membrane; B corneas swelled lightly and some ruga emergied in its. 48h: A the same situations as in 24h; B eyeballs softened, anterior chambers shallowed ,corneal opacity appeared, more ruga emergied in its. Quantity of endothelial cells reduced sharply and many black sites appeared, compared with A difference was obvious (P<0.01).(72,96)h: A still transparent with little edema(96h) and few ruga in its. Quantity and appearance of cells stable. B eyeballs softened deeply to sinking. cornea opacity serious. Couldn't look clearly into anterior chambers and calculate endothelial cells. C (24,48)h:corneas transparent without obvious edema. But the reflection of light from air bubbles too violent to image information. Compared the percentage of vital cells in the same duration. A was good; C was worse; B is the worst. The difference was obvious (P<0.01). Corresponding to different moment, comparatives still obvious (P<0.01).(2)Midterm preservation: 3 day: corneas all transparent in group Ⅰ,Ⅱ,Ⅲ. A little edema in the contrast. (5,7)d, a little edema in groupⅠ,Ⅱ,Ⅲ,much in the contrast with transparence disappearing. Compared during same period, corneal transparence was the best and edema was the least in groupⅠ. The percentage of vital cells with trypan blue-alizarin red staining in groupⅠwas the highest , and in the contrast was the lowest. Between any two groups, differences all obvious (P<0.01). Compared average trouslucent density with SDH and G-6-P enzyme staining ,difference between any two groups all obvious (P<0.01). Along with the course, vitality of endothelial cells generally decreased. Difference to the compared moments was obvious (P<0.01). In TEM and SEM, between groupⅠand contrast, compared respectively the ultrastructure at (3,5,7)d,difference of appearance and substructure integration was obvious.Conclusions:In cornea preservation, SH can succeed in protecting and stabilizing corneal endothelial cells. In short-term and midterm course, usingSH could greatly improve the quality of cornea and prolong the duration. How efficiently SH acts in midterm preservation was related to its consistence in the liquids...