| Objective: Periodontal diseases are considered as infection of the periodontium due to subgingival pathgens. The characteristic is the infection of opportunistic pathgens. Porphyromonas gingivalis (Pg), Provotella intermedins (Pi) and Fuso-bacterium nucleartum (Fn) are believed very important for the emergence of periodontal diseases, They can produce lots of virulence factors such as endotoxin, proteas, ammonia, indole, etc. These virulence factors can destrust periodontium and caus periodontal diseases. The control of plaque is the key to periodontal therapy. There were widely study in the use of antibiotics and antimicrobials in the therapy. Systemically or locally administered bacteriocidal drugs had significant effects on inhibiting periodontopathogens. But the Systemical use of antibiotics and antimicrobials for a long time would lead to unpleasant side effects such as dysbacteriosis, the reaction of gastrointestinal tract. Therefore, the locally administered bacteriocidal drug in the therapy was more and more important. Chinese herbal medicine have been used for a long time. Many Chinese herbal medicines have exhibited strong inhibition on periodontopathogens. So, there wasincreasing emphasis on researching safe and effective Chinese herbal medicines locally administered in periodontal pockets. Shuanghuangbu was a new double-layered local Chinese herbal delivery device, which mainly contained Rhizoma coptidis, Radix scutellariae and Rhizoma drynariae. We have found the Shuanghuangbu could significantly improve the clinical symptom of periodontitis and might change the composition of subgingival microflora at the disease sites to that at the healthy sites. The purpose of this study was using the liquid media two-fold dilution method and the digging holes agar diffusion method to research the effect of Shuanghuangbu decoction, Rhizoma coptidis decoction, Radix scutellariae decoction and Rhizoma drynariae decoction on Porphyromonas gingivalis, Provotella intermedins and Fusobacterium nucleartum. Methods: A mixture of Rhizoma coptidis, Radix scu-tellariae and Rhizoma drynariae was soaked in water for 30 minutes , boiled for 45 minutes and filtered; The residue was reboiled with water and refiltered. All decoctions were mixed, heated and concentrated to 1:1 Shuanghuangbu decoction ( 1ml Shuanghuangbu decoction made from 1g of raw material ). The Millex-GP filter was used to filter sterilization the Shuanghuangbu decoction. Rhizoma coptidis decoction, Radix scutellariae decoction and Rhizoma drynariae decoction were made in the same methods. Porphyromonas gingivalis, Provotella intermedins andFusobacterium nucleartum were chosen as the experimental bacteria. These periodontopathogens were cultured on Schaedler broth agar culture medium or in Brain-heat infusion broth. The liquid media two-fold dilution method was used to measure the minimum inhibitory concentration ( MIC ) of Shuanghuangbu decoction, Rhizoma coptidis decoction, Radix scutellariae decoction and Rhizoma drynariae decoction on Porphyromonas gingivalis, Provotella intermedins and Fusobacterium nucleartum. Schaedler broth was used to dilute 2-fold the Chinese herbal medicines decoction in the test-tubes. The final concentration of the drugs ranged from 1:2 to 1:1024. Two test-tubes that only contained Schaedler broth were used as a positive control and a negative control, respectively. Periodontopathic bacteria to be used in the MIC test was added to the test test-tubes and the positive control test-tube, finally, the bacteria concentration of each test-tube must became to 1×106 CFU/ml. The test-tubes were then incubated anaerobically at 37℃ for 48hr. The MIC was taken as the lowest concentration of the drugs that completely inhibited growth. The digging holes agar diffusion method was used to measure the diameter of inhibition ring of Shuanghuangbu decoction on Porphyromonas gingivalis, Provotella intermedins and Fusobacterium nucleartum, 2% organidin was chosen a control drug. Made four Schaedler broth bloo... |
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