Research On Microanalysis Method Of Sulfated Polysaccharide Drug 911

911 is a marine sulfated polysaccharide drug with good anti-HIV activity, which is derived from brown algae.The physical and chemical characteristics of 911 are analyzed systematically. The molar ratio of sulfate ions to carboxylate ions is determined by conductometric titration method and the molecular weight is tested by high performance gel permeation chromatography(HPGPC). The precision of these two methods is also studied. To improve the detection sensitivity, guanidine hydrochloride is used as the best derivation reagent of 911. A systematic research is done on the optimum derivatization condition of 911 and Guanidine hydrochloride. The M /G ratio of 911 has direct relation to its biological activity. Post-column fluorescence derivatization is successfully applied to determine the M/G ratio of 911.The method is simple, sensitive and the precision is 0.4%, better than UV detection of HPLC. There is no appropriate microanalysis method for 911 because of the sensibility limitation of the known quantitative detection methods.In this paper, post-column fluorescent derivation HPLC is firstly used to analyze 911, the detection limit can reach ng level, standard curve and linearity range are determined. This microanalysis method is also roughly used to analysis 911 in the serum .The research results of 911 can be applied to its quality control. Post-column derivatization method provides the basis of pharmacokinetic studying by chromatography.