| The phospholipid is main, composition of membrane structure of human body and all animals and plants'cell. The membrane phospho-lipids include mainly sphinyomylines ( SM) , phosphatidyl choline (PC) , phosphatidyl ethamlolamine ( PE ) , phosphatidyl serine ( PS). The content of every phospholipids is in a certain proportion and their distribution are regular. In recent years, researchers have found gradually that the change of composition of the mrmbrane phospholipids and distribution related to many kinds of diseases and the change of cells function, so more and more scholars are paying attention to it.The abnormal change of phospholipids of erythrocyte membrane usually means the body is attaced by disease. At present, many studies have indicated the change of erythrocyte membrane phospholipids occuring in the patients with many kinds diseases , but there were few studies about the relation of cerebral infraction and erythrocyte membrane phospholipids. The infraction disease is a common disease, frequently - occurring disease. It is very high incidence in our country and American - European countries, specially a increasing tendency rapidly is shown in our country in recent years. A large amount of research work indicates that the infraction disease is the disease of more than one kind of cause. The red cell accounts for 45% in the blood , its role during the course of thrombosis should not be ignored . In thisstudy ,we determined the contents of TPL , every composition of e-rythrocyte membrane phospholipidsand membrane CHO in case and control groups to investigate the relation of phospholipids of erythrocyte membrane , CHO of membrane , plsma lipids and the mechanism of cerebral infraction.Materials1. Study subjectsThe case groups are 42 patients ( 22 males, 20 females, mean age; 65. 3years) with cerebral infraction confirmed by head CT arid MRI from neurphogic department of LiaoNing Province Peoples Hospital; 40 control subjects ( 20 males, 20 females, mean age; 66. 5 years). All subjets exclude cardiovascular disease , liver and kidney disease and diabetes mellitus; have not use antioxidation or decrease lipids drugs recently.2. Collection of Blood SampleA 4ml venous blood sample was collect from patients in 24 hours after cerebra infraction and controls after an overnight fast and was drawn into tube containing heparin sodium. All samples were prepared by centrifugation at 3000r/min after collection. Plasma samples were stored at -70℃ until used. 0. 5ml RBC was collected in a tube with cap and stored at -70℃.Methods1. Method and ReagentsThe content of every component of erythrocyte membrane was measured by thin layer chromatogram; the contents of CHO and TPL of erythrocyte membrane were measured by colorimetric; plsma TG ,CHO and HDL - c were determined by enzymekits supplied RAN-DOX; LDL - c was measured by immune turbidimetry.2. Main instrumentsOLYMPUS AU1000 Biochemistry Analyzer in Japan; UV spec-trophotometer, UV -265 FW in Japan; thin layer scan meter in Japan.3. Statistical AnalysisThe results were calculated as mean pules or minus one standard deviation. Statistical analyses were performed by Excel software. The analyses of difference were performed by t - test. A value of less or e-qual to 0.05 was considered significant. Correlation coefficients were determined by the pearson test and from linear correlation analysis.Results1. The comparison of the membrane total phospholipid , phos-pholipid component and membrane cholesterol between case and control was seen table 1. There are significant differences between cerebral infraction patients and healthy controls for PC(0. 60 ±0.09vs 0. 76 ±0.14 mmol/L ,p<0. 01) , PE(0. 68 ±0.04 vs 0. 72 ±0. 12 mmol/L ,p<0.05), TPL(2. 39±0.45 vs 2. 56±0.12 mmol/L ,p<0.05), CHO(4.61 ±0.11 vs4.27±0.08 mmol/L ,p<0.01), CHO/TPL( 1.94 ±0.26 vs 1.54 ±0.23,p <0. 01) , SM/PC(1.27±0.42 vs 0. 91±0.22,p <0.01) , though SM was higher, there was no significant differences.2. The comparison... |
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