| ObjectsPeritoneal carcinomatosis is mostly metastasized from hepatic, gastric, colonic, and ovarian carcinoma. It is the mark of advanced neoplasm, and one of main reasons which neoplasm patients die from. Contrast to systemic chemotherapy, intraperitonial chemotherapy can increase the concentration and total amount of chemotherapy agents in peritoneal cavity, prolong the contact time between agents and foci, diminution the systemic side effects. As a result, intraperatoneal chemotherapy is widespreadly applied in clinic. We compared the effect of intraperitoneal chemotherapy in high - dose with in successive low - dose and observed the drug dynamic change of cisplatin ( cD-DP) absorbed in tumor and in blood, and investigated thesustained -release of cisplatin embodied in fibrin glue ( FG - cDDP) and its curative effect on mice with peritoneal transplant carcinoma. Then we discussed the new methods of treatment on peritoneal carcinomatosis.Experimental Materials and Methods1. Animals; C57BL mouse, female, 18-22g.2. Cells; B16 melanoma cells3. Instrument: atomic absorption spectrophotometry ( HITACHI 180-80).4. Drugs; Fibrin Sealant or Fibrin Glue (FG) , cisplatin.5. B16 melanoma cells were routinely subcultured in laboratory, then cell suspension was made.6. Peritoneal transplant carcinoma model with C57BL mouse was set up by inoculating 8 x 105/0. 2ml B16 melanoma cells per mouse.7. Experiment on thesustained - release of cisplatin embodied in fibrin glue in vitro; FG is composed of liquid A and B. We put cDDP 5mg in liquid B, and sprayed two liquids on bottom of Kolle flask simultaneity, then the solid glue was formed. We added nature Saline 5ml on glue, then took supernatant to stay for checking on 1, 2, 4, 8, 12, 24, 48, 72, 96 hours later.8. Treatment experiments on transplant carcinoma; 96 mice bearing melanoma were divided into 4 groups at random, each group is composed of 24 mice. Group 1 is control (group NS) ; each mouse was injected intraperitoneal with NS 0. 2ml. Group 2 is group cDDP embodied in FG (group FG - cDDP) ; Laparotomy was operated on each mouse via left upper quadrant, then FG - cDDP 0. 2ml was sprayed on epiploon, doses of cDDP is 12 rag/kg each mouse. Group 3 is group high - dose cDDP ( group cDDP) ; each mouse was injected intraperitoneal with cDDP, doses of cDDP is 12 mg/kg each mouse. Group 4 is group successive low - dose cDDP ( group cDDP x 3 ) : each mouse was injected intraperitoneal with cDDP continuous in three days, doses of cDDP is 4 mg/kg each mouse per times. Mice were put to death in the 1st, 2nd, 3rd and 6th day after intervene, and numbers of neoplasm node on peritoneal were counted. Each time 6 mice werekilled in each group. Meantime epiploon and peripheral blood was taken to stay for checking. Epiploon was stored in -40℃, and blood in4℃.9. Assay of platinum content: The content of platinum was measured by atomic absorption spectrophotometry. Tissue was disposed by nitric - perchloric acid moist decompose pattern, peripheral blood can be assayed directly.10. Statistic software SPSS10. 0 was adopted in statistics analysis. Every parameters were showen as average mean ?standard difference ( x + s). Average mean was compared with independent sample t - test.Experimental Results1. Black neoplasm node on peritoneal cound be seen macrography 48 hours later after melanoma cells were injected intraperitoneal. The mice bearing melanoma and the ones operated beforehand survived o-ver 2 weeks.2. In vitro, FG block cant dissolve until the 4th day. 50% dose of cDDP was released from block in 2nd hour, nearly 80% in 24th hour, and maintained a higher lever in 3rd, 4th day.3. Result of treatment on mice bearing melanoma1) Contrast to control, the melanoma node numbers of group 2, 3, 4 decrease sharply in 2nd , 3rd, and 6' day after intervene, which has statistic meaning.2 ) The melanoma node numbers of group FG - cDDP is less than group cDDP in 3rd and 6th day after intervene, which has stati... |
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