| Methods: Hepatoma cell lines BEL-7402 and SMMC-7721 were treated with different concentrations of As2O3 in combination with various time; viable cells were determined by trypan blue exclusion; apoptotic cells with Gimsa staining were observed under microscope and agar electrophoresis was used to assay DNA from apoptotic cells, and DNA contents of apoptotic cells were determined by flowcytometry; telomerase activity of cells treated by As2O3 was assayed by Telomerase PCR ELISAPLUS Kit and GSH contents of them were detected so as to compare the sensitivity between them to As2O3.Results: From 0.25 to 2.00μmol/L, As2O3 can inhibit growth of BEL-7402 cells significantly with concomitant of apoptosis, increasing sub-G1 phase cells, and decrease its telomerase activity, which showed dose-dependent and time-dependent effects. There is no any effect of As2O3 on SMMC-7721 cells at level of 0.25μmol/L. However, there exist effect of As2O3 mentioned above at level of 0.50 to 2.00μmol/L. The experimental result also showed that GSH involved in sensitivity of BEL-7402 and SMMC-7721 cells to As2O3, the higher level of GSH content in cells, the lower sensitivity of cells to As2O3.Conclusion: As2O3 can inhibit hepatoma cell lines BEL-7402 and SMMC-7721 cells growth, the mechanisms perhaps are to induce apoptosis and to decrease their telomerase activities. The GSH content can effect the sensitivity of them to As2O3.
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