Expression And Significance Of P27, Cyclin D1 And Cyclin E In Hepatocellular Carcinomas

Objective To study the expression and its significance of P27,cyclin D1 and cyclin E protein in hepatocellular carcinomas(HCC).Methods Expression of P27 cyclin D1 and cyclin E protein were investigated by immunohistochemistry technique(SP) with 50 hepatocellular carcinomas who had undergone surgery for HCC ,as well as 22 of their adjacent noncancerous lesions and 9 normal liver control specimens. All of the antibodies employed for immunohistochemistry were from MaiXin company.Sections for negative control were prepared by using normal mouse serum instead of primary antibody.Results The average P27 LI in hepatocellular carcinomas were 49.38±37.22％,which was higher in HCC lesions than in the adjacent noncancerous lesions(34.55±22.76％ P＝0.002). Furthermore,the LI in adjacent noncancerous lesions was significantly higher than in normal liver lesions(14.33±12.00％,P＝0.007). P27 LI was significantly decreased in case with portal invasion(P＝0.018),poor differentiation(P＝0.005),larger size (P＝0.032 ), intrahepatic metastasis(P＝0.008)and higher stage(P＝0.014). Overexpression of cyclin D1 and cyclin E in adjacent noncancerous lesions and normal liver lesions have not been found. The cyclin D1 and cyclin E overexpression were observed exclusively in HCC lesions. Cyclin D1 overexpression was 34% of the cases and occurred more frequently in those with poor differentiation (P＝0.029)and intrahepatic metastasis(P＝0.023). Cyclin E overexpression was 40% of the cases and occurred more frequently in those with poor differentiation (P＝0.009)and intrahepatic metastasis(P＝0.001 ). Conclusions These findings suggest that in HCC P27 is active against HCC progression in early phases and may be helpful in determing tumor progression. Overexpression of the cyclin D1 and cyclin E were helpful in determing the aggressiveness of hepatocellular carcinoma,and were closely related to occurrenc and progression of hepatocellular carcinoma.