| Objective: For a period of time, we have been repairing bone defects created from wound, tumor, infection or other causes by the way of autograft of bone. But the source of autogenous bone is limited to place , range, and quantity in the cutting materials, autogenous bone can't sometimes meet clinical needs. Therefore it is the main object for many scholars to search for other live transplantation materials to repair bone defect. So author have undertaken the experimental study on animals on the basis of predecessor's research achievement, the purpose of the study is to observe if autograft of periosteum wrapped up in collagen membrane compounded by staphylococcus aureus injection can guided bone regeneration, another purpose of this study is to explore the mechanism of guided bone regeneration. On these grounds we search a new method of bone defect therapy.Method: 60 adult New Zealand rabbits were divided into 3 groups of 20 animals each at random. They were operated into standard bone defect experimental models in the middle part of amphi-radius by resecting 1.5cm radius bone. GroupA were filled with collagen membrane/periosteum/ staphylococcus aureus injection; Group B were filled with collagen membrane/staphylococcus aureus injection only; Group C were filled with periosteum/staphylococcus aureus injection only. Experimental animals were sacrificed at 2rd, 4th, 6th, 8th and 12th week postoperatively, 4 animals of each group were sacrificed each operation. After muscle were removed away from the specimens, results were revealed in gross observation, histology and X-radiography. Moreover, level of alkaline phosphatase, serum calcium and bone gla protein were measured on each group animals. By the score of X-rays carried through in according to the condition of bone regeneration, bone defect's connection and bone shape, and the statistics treatments of alkaline phosphatase, serum calcium and bone gla protein, the effect of bone defect repaired with autograph of periosteum wrapped up in collagen membrane compounded by staphylococcus aureus injection has been evaluated objectively.Result: Gross observation, histological analysis, X-ray films and biochemical examination were taken at 2rd, 4th, 6th, 8th and 12th week postoperatively. The results were following: In group A, there were distinctive periosteal reaction and lots of callus growth in transplantation area, consistent callus finally bridging the gaps of defect. New bone proliferated in the way of intramembranous ossification,mature bone and bone marrow cavity were gradually formed, bone defect healed completely. In grop A and part of group C, there were not distinctive periosteal reaction and bone callus in transplantation area in contrast with group A. The defect was occupied by surrounding connective tissue, it made the process of new bone growth and mature bone replacement delay. The show of others of group C were similar to group A. Results of histological observation reflected: In goup A, during the application period, collagen membrane had no evidence of displacement or collapse, it showed no foreign body or rejection reaction, it began to degrade at 6th week, entirely degraded at 8th week, degradable products did not destroy bone regeneration process. New bone remarkably had been completely healed. In group B and part of group C, new bone growth and mature bone replacement were slow owing to ingrowth of connective tissue in transplantation area, small vessel proliferated unobviouslly. The show of others of group C were similar to group A. Results of immunohistochemical examination reflected: In group A, BMP positive distributed in osteocytes of bone ends, proliferating periosteum, medullary cavity and endosteum. Intramembranous ossification predominated in area of defect. In group B, BMP positive distributed mostly in matrix of cartilage and slurry of cartilage, and entochondrostosis predominated in area of defect. In group C, BMP positive distributed mostly in periosteum of bone ends. Moreover,distribution of BMP wasn't even in... |
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