The Animal Experiments Of B→O Blood Conversion

Objective: To identify human ABO-liked blood groups on red cells of cynomolgus monkey living in southern China. To investigate the enzymolysis effect of a -galactosidase on human B-liked antigen of cynomolgus monkey red cells as well as on changes of red cells in morphology and function. To analyze alterations of components and concentrations in blood and urine after enzyme-treated blood transfusion and evaluate the security of this treatment.Methods: Two with A and three with B human-liked blood groups cynomolgus monkeys were selected by modified absorption-elution test from total mumber of 30 individuals. Red cells with human B-liked antigen were treated by recombined α-galactosidase and the affect of enzyme-treatment was evaluated by absorption-elution test. Morphology changes were observed by optic and electron microcope and membrane fragility, autohemolysis, cholesterol, methemoglobin, acetylcholin activity, ATP, 2,3-DPG and P50 were detected for evaluating membrane integrality, metamorphosis, oxygen, and so on. The a -galactosidase treated red cells were labeled with FITC and then infuse to another blood group monkey (B1→Al,B2→A2, B3→B3) and in the meanwhile record their blood pressure, pulse and respiration. Flow cytometer was used to trace donor red cells metabolism in recipient blood. The component and concentration of recipient blood and urine were analyzed at different time.Results: The blood groups in 30 monkeys were distributed as 3 of group A (10%), 9 of group B (30%), 3 of group AB (10%). The human B-liked antigen on cynomolgus monkey's red cells could be eliminated by α-galactosidase enzymlysis and showed a negative result when detected by modified absorption-elution test . α-galactosidase enzymolysis did not make any changes in morphology membraneintegrality and its normal function. The blood pressure, pulse and respiration of recipients post transfusion were not significant change compared with pre-transfused. Viabilities of a -galactosidase treated red cells within 24 hours were 84.6% and 68.1% respectively versus 88% in control, Ti/2 were 7d and 8d versus 13d in control. Components and concentrations in recipient blood and urine did not show a significant change compared with their control(P>0.05).Conclusions: Human ABO-liked blood groups antigens exsisted on the red cells of cynomolgus monkeys of Southern China. Human B-liked antigen could be enzyme-treated by a -galactosidase. Enzymolysis by a -galactosidase showed no effects on red cells of cynomolgus. Transfusion of enzymolyzed human B-liked red cells into human A-liked cynomolgus monkeys showed no significant difference in blood and urine (P>0.05).