| ObjectTo investigate the protective effects of anti-tumor necrosis factor- a monoclonal antibody(anti-TNF-alpha mAb) on rat renal ischemia-reperfusion injury.MethodsFifty normal male Sprague-Dawley rats, weighting 195-205g, were randomly divided into 3 groups. Group A (treatment group) was subjected to ischemia-reperfusion injury with intravenous administration of anti-TNF-alpha monoclonal antibody(0.1mg/kg) 5 minutes before reperfusion. Group B(non-treatment group) with the same injury followed by saline administration in the same manner, and group decontrol group) with only anesthetization and leparotomy but not ischemia. Routine assays were performed for testing the levels of blood creatine(Cr), blood urea nitrogen (BUN),plasma TNF-alpha and the renal cell apoptosis. Ultrastructure of the kidney was also observed.ResultsRenal ischemia-reperfiision resulted in significant increase of serum Cr, BUN and TNF-alpha(p<0.01), but the damage were prevented by administration of anti-TNF-alpha monoclonal antibody before reperfusion(p<0.01). In group B, widespread pathological changes and cell apoptosis were observed in the renal tissue following ischemia-reperfusion injury, while similar changes were scarcely visible in Group A.ConclusionRat renal ischemia-reperfusion injury can be attenuated by administration of anti-TNF-alpha monoclonal antibody before reperfusion. |
PDF Full Text Request