Expression Of Insulin-like Growth Factor-I(IGF-I) And Insulin-Like Groth Factor-I Receptor (IGF-IR),Estrogen Receptors(ER),Progesterone Receptors (PR) In Uterine Leiomyoma

Objective to study the expression of insulin-like growth factor-I (IGF- I ) and insulin-like growth factor- I receptor (IGF- I R), estrogen receptor(ER) and progesterone receptor (PR) in the uterine leiomyoma and to investigate the pathogenesis of uterine leiomyoma. Methods Immunohistohemical method (Strepavidin-Peroxide) was employed to detect the expression of IGF- I , IGF- I R,ER and PR in both uterine leiomyoma and myometrium(as normal control) of patients,and flow cytometry (FCM) was performed to ensure the exactly expression value of what was to study.Result 1.IGF- I were overexpressed in the uterine leiomyoma and there was a significant higher SP positive stained in uterine leiomyoma than that in normal myometrium. positive cell rate was 80% to 40%(P<0.05). statistical difference was significant. flow cytometry result of IGF-I in the uterine leiomyoma (6.24 ± 1.46) was higher than control class (5. 58±1. 07, P<0. 01). 2. The staining of IGF- I Ra and IGF-I 3 were stronger in uterine leiomyoma than that in myometrium(P<0. 01), and IGF- I R expression was in high level in FCM (7. 39 ± 0. 54, P<0. 01), the FCM value of myometrium was (5. 14 ± 0. 51) 3. the result of ER and PR in the uterine leiomyoma were higher than that in normal myometrium (P<0.05,OP<0. 05) , and the expression of IGF- I in uterine proliferation phrase was positive corelated with expression of ER(r=0.832,p<0. 01)Conclusions 1. uterine leiomyoma have the capacity of secretion IGF-I and have higher expression than the expression of IGF- I in theuterine smooth muscle cells. IGF- 1 improve the growth of uterineleiomyoma in short time by enhance the mitosis of the cells, which playan important role in the pathogenesis of this tumor.2. IGF-I receptor are overexpresssd in the uterine leiomyoma than in the myometrium, which not only promote hormone uptaking, information convection, but also have effect on uterine smooth muscle conversion and uterine leiomyome formation.3. IGF- I further improve uterine leiomyoma growth through introducing the action of sex hormone. IGF-I act as a mediator of estrogen, enlargement the proliferation signal of estrogen by auto/paracrine pattern. IGF- I also enhance cells mitosis and growth of uterine leiomyoma indirectly by regulating progestin synthesis.