The Experiment Research Of Bone Formation Protein-2 Expression In The Interface Of Ti6Al4V Alloy Disposed With Surface Oxidation Membran

ObjectiveTo further the expression of the bone formation protein -2( BMP-2) in the interface of TJ6A14V alloy disposed with surface oxidationmembran N stainless steel and titanium alloy ( Ti6A14V) , and to studythe biocompatibility and ability of osseointegrate of the three implantsmaterials, supply the experimental data for the clinical application.MethodsThere are 90 rats were used and divided into three groups according to the three materials, eachly 30 in each group, 15 of each sex. The three materials were made into a cylinder, the diameter 3mm , thickness 1.0 mm and planted in outside oblique line of mandibular of rats. After 1,2,3,4,6 weeks gete material, fix, dycalcium , then the HE and BMP - 2 immunohistochemical staining and image analysis system in order to analyze the average gray value. Comparison with Ti6A14V and stainless steels.ResultsHE staining showed Ti6A14V alloy disposed with surface oxidation membran arrived an earlier osseointegrate than Ti6A14V and stainless steel. BMP - 2 staining showed the average gray value of the three materials arrived the top in the second week, but the average gray value of Ti6A14V alloy disposed with surface oxidation membran were higher than the other two materials (p <0.05). In the next few weeks , there were declining of average gray value of the three materials , but Ti6 A14 V alloy disposed with surface oxidation membran were higher definitely than the two others ( P < 0.05 ).ConclusionThe biocompatibility and osseointegrate ability of Ti6A14V alloy disposed with surface oxidation membran were better than Ti6A14V and stainless steel.