| Romulus cinnamomi is known as an antiviral agent and used for treatment of cold and women's diseases. 43 samples of Ramulus cinnamomi were collected from different areas. First.the HPLC method was established to determine the contents of cinnamaldehyde ,cinnamic acid ,coumarin and cinnamic alcohol in Ramulus cinnamomi .The linear ranges of cinnamaldehyde.cinnamic acid ,cinnamic alcohol and coumarin were 0.208 ~ 3.33 g ( r=0.9997 ) , 0.100~1.60ug (r=0.9999),0.204~3.26ug(r=0.9999), 0.0674-1.08pg(r=0.9999), The average recoveries were 98.3%(RSD=0.95%), 98.0%(RSD=1.4%), 99.1%(RSD=1.2%), 98.3% (RSD=1.8%), respectively. Second , protocatechuic acid was determined by HPLC , It had a good linearity within the range of 0.0464~0.557(ag (r=0.9999) . The average recovery was 98.1% (RSD=1.1%) .In this paper , the chemical constituents in the methanol extract of 43 samples of Ramulus cinnamomi were analysised by HPLC. 10 original chemical features as a whole were obtained . 43 sample were devided into three grades -the superior, the ordinary and the fake by Hierarchical clustering analysis. Based on the chemical data, an unknown sample can be classified precisely by this method. Thus it was possible to assess the quality of Ramulus cinnamonani only using chemical data.KEY WORDS : Ramulus cinnamomi;HPLC;Figureprint;Chemical pattern recognition;Quality assessment...
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