Preliminary Study About Effects Of Stem Cell Factor On Female Reproduction Process

With the development of scientific technology, there have been a great deal of process in infertility and research in its cause and treatment, which is related to study about female reproduction process. It has been confirmed that there is as intraovarian regulative factor which control female reproduction process not only the hypothalamic-pituiary-ovarian axis(HPOA) and uterus. And there is other cytokine system or network which control follicle production and development, ovulation and oocyte maturation, fertilization and cleavage and pregnancy.It has been demonstrated that stem cell factor closely assiociated with reproductive function in the regulative network, so much attention have been paid to study the ovarian function by scientist.Object: To investigate the changes of stem cell factor(SCF) in ovarian hyperstimulation cycles and its effects on human oocyte in vivo. The aim of this study is that try to explore the rolesof stem cell factor in different levels. Part I: The changes of SCF and female sex hormone in ovarian hyperstimulation cycles; Part II : Effects of SCF on oocyte maturation, fertilization and cleavage in vivo; Part III: Serum levels of SCF in different pregnant period.Methods: (1) 40 of women in vitro fertilization and embryo transfer(IVF-ET) who received induced ovulation by the same method as routine. Age, days of menstrual cycle, body mass index(BMl), number of follicle, days of follicle development, days of eggs retrieved and total number hMG used were recorded. ELISA and RIA were used to determine the levels of SCF in follicular fluid(FF) during oocyte pick-up and in serum before and after superovulation, and the levels of E2, P and T in FF during oocyte pick-up were measured by RIA. (2) Total cycles which were oocyte maturation, fertilization and cleavage of the 40 IVF-ET patients were observed and recorded in vitro through invert microscope. (3) ELISA were used to determined the levels of SCF in serum of patients who were 100 in infertility and 150 in different pregnant period.Results: (1) ①Total FF SCF levels were significantly higher than those of matched serum levels during Opu, FF SCF levels were the same in high an middle groups, not in low group (P <0. 05) . ②The levels of SCF in FF among 3 groups were different, the low group were lower than other groups (P<0. 01) .③There were no significant changes of serum SCF level in different groups and before and after superovulation (P >0. 05) . ④FF SCF levels were positively correlated with number of developing follicles (r=0.991, P <0. 01). ㏒erum levels of FSH and LH werenot significantly different in three groups (P >0. 05). FF FSH and LH levels of low reactive group were lower than those of the others (P0. 05). Rates of fertilization and cleavage in low group were lower than those in others (P<0. 05); ③uneven cleavage and cell piece were different in three groups between them cultured for 36 hours and 48 hours (P< 0. 05) . Oocyte would die such as stopping to be mature,to be fertilized and cleaved.Conclusion: (1) SCF was produced by developing follicle itself, not permeated from blood, and it may play a complementary role in regulation of follicle development.(2) SCF and gonadotropins may have coordinated effect on development of follicles during gonadotropin stimulation in IVF-ET program. (3) SCF may promote oocyte maturation, fertilization and cleavage in vivo, which can increase the rate of pregnancy, and affect pregnancy.