| BackgroundCongestive heart failure is the end stage of all kinds ofcardiovascular diseases. The process of coronary heartdisease involves stricture of coronary blood support,myocardiocyte losing and heart failure. The death reason ofmyocardial infarction most common is heart failure, which causeby myocardiocyte losing. Some study showed that transplantedsmooth muscles , skeletal myoblast cells and cardiomyocytesshould limited scar expansion and prevent onset or developmentof heart failure, but these cells were eventually eliminatedby rejection. How we can treat a "broken heart" ? Many kindsof cells can be use to repair the infracted area. Bonemarrow-derieved mesenchymal stem cells (MSCs), is the mostexpectable candidate. Because it is one kind of stem cells, hasself-reflash and multipotential power to other tissue, such asmuscular, nervous tissue. Recently some research showed aftertransplantated into the myocardial infarction region, the MSCscould transdifferent into cardiomyotes and improve heartfunction. This study treat the heart infarction model of NewZealand white rabbits by implantation of agnate MSCs toinfracted area.AimTo conform if the MSCs could repair the damaged area, if those cells could live in infracted area, if those cells could divide into myocardiocytes, if those cells could form vessels, if those cells could improve function of infracted heart.MethodsThe male New Zealand white rabbits, was divided into 3 groups. The group one is treated as heart infarction model, after then MSCs is injected to the heart infracted area. The group two is the group of comparisons, should be inject PBS to compare the MSCs. The group 3 is sham group.Before 48h of MSCs implant, the MSCs should be marked by Brdu in culture plate. Before 24h of operation all the animals of three groups were given Cyclosporin A Oral Solution 15mg/kg , once a day for 4weeks post-operation. Ecocardiography was given in each group per-operation and post-operation 4 weeks. The hearts were harvested for HE, anti-BRDU and anti-c troponin I immunohistochemistry pathological examination. To check if there were some Brdu(+),cTn I (+) cells in the infarction area. ResultsEF of MI+MSCs group (0. 64 + 0. 07) were higher than MI group (0.52+0.09) after 4 weeks (P<0. 05). The transplanted cells keep life in infracted area, some of them different to cardiomyocytes, some of them to vessel tissue, and others to fibrocytes. ConclusionsAfter transplanted into the myocardial infarction region, the MSCs could transdifferentiated into cardiomyocytes and induced regeneration of vessel structures, improved cardiac function. MSCs transplantation could improve heart function in heart infarction model of New Zealand white rabbit. |
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