Study On The Relationship Between Epstein-Barr Virus And Nasopharyngeal Carcinoma

Objcctive To study the Epstein-Barr virus DNA distribution inparaffin-embedded tissue, fresh tissue, peripheral blood leukocyte and serum innasopharyngeal carcinoma patientsnyPC). We also study the relationship between NPCand EBV It is useful for making diagnosis and differential diagnosis of thenasopharyngeal carcinoma by polymerase chain reaction. We can further understand therelationship between NPC and EBV by molecu1ar biology way.' Method Using polymerase chain reaction method, 39 patients with NPC(including 25poorly differentiated squeaxnous cell carcinoma, l0 well differentiated squeamous cellcarcinoma, l vesicular nucleus ce1l carcinoma, 1 nasopharynx basal celladenocarcinoma, l nasopharynx mucoepidermoid carcinoma, l nasopharynxcystadenocarcinomMxperimental group-1)20 patients with chronic nasopharyngitis(controI group-1) were tested fOr the presence of EBV DNA in paraffin-embeddedtissue. We also measured 24 patients with NPC(including l0 undifferentiated carcinoma,7 poorly differentiated squeamous cell carcinoma, 6 well differentiated squeamous cellcarcinoma, 1 vesicular nucleus ce11 carcinoma-- experimenta1 group-2) and 10 patientswith other head and neck carcinoma (6 carcinoma of larynx, 3 maxillary sinuscarcinoma, 1 lymphomaxiontrol group-2.l) and l0 patients with chronicnasopharyngitis-ontrol group-2.2) were tested for the presence of EBV DNA il1peripheral b1ood leukocyte and serum. We measured 3 fresh tissue with NPC and the 3fresh tissue were included in the experimental group-2. At the same time, we alsomeasured EB virus VCA-IgA antibodies with enzyme-immunoassay method inexperimental group-2 and coniro1 group-2. l; 2.2 in serum.&4#Z4+kqsn+#{a{tkResult The resu1ts showed that 28 of 39 nasopharyngeal carcinoma patients (7l .8%)and 3 of 20 control group-l(l5%) were EB virus DNA positive in paraffin-embeddedtissue. The difference between experimental group-l and control group-1 wasstatisticaIly signiflcant (Exact xz Test, P<0.0l). In experimental group-2, 10 of 24nasopharyngeal carcinoma patients (4l .67%) and l of 10 control group-2.1 (l0%) wereEB virus DNA positive in peripheral blood leukocyte. control group-2.2 EB virus DNAwas negative in peripheral blood leukocy'te. The difference between experimentalgroup-2 and control group-2.1 was not statistically significant. But the differencebetween experimental group-2 and control group-2.2 was statisticaIly significant (Exactxz Test, P<0.05). In experimenta1 group-2, 3 fresh tissues, 2 of them were EB virus. positive. But in group-2 the EB virus DNA was negative in serum. 2l of 24 VCA-IgAantibodies were positive(87.5%) in experimental group-2. In control group-2.1 andcontrol group-2.2 VCA-IgA antibodies were positive 20%, 0% respectively. Thedifference between experimental group-2 and control group-2.l was statisticallysignificant, and the experimental grouP-2 and control group-2.2 was statisticallysignificant too(Ridit Test, P<0.01 ).ConcIusion The results suggested that EB virus was decrease from tissue toperipheral b1ood leukocyte. In serum the EB virus DNA copies are too weak to bedetected. There must be some relationship betweel1 VCA-IgA and EB virus DNA inperipheral blood leukocyte.