Expression Of PTEN And CDK4 In Human Endometrial Carcinoma And Its Clinical Significance

Objective: To study the relationship between cyclin dependent kinase 4 (CDK4),PTEN protein expression and carcinogensis,progression and recurrence of endometrial carcinoma .Methods:A specifically and sensitively immunohistochemical S-P assay was used to detect the expression of PTEN and CDK4 in paraffin-embedded sections of 50 cases of endometrial carcinoma, 12 atypical hyperplasia of endometrium and 10 normal endometrium.Result:The positive immunostainning rate of PTEN in endometrial carcinoma, atypical hyperplasia of endometrium and normal endometrium was 56.00%,91.00%, 100.00%, respectively;and the difference was statistically significant between carcinoma and normal endometrium or atypical hyperplasia of endometrium(P<0.05).The expression of PTEN correlated with muscles invasion (P<0.05),but not with clinical stage and histological grade or recurrence. The CDK4 nuclear positive rate was higher in atypical hyperplasia of endometrium than that in normal endometrium (P<0.05). The positive rate of nuclear expression in cancer was significantly higher than that in normal endometrium (P<0.05). There was a difference between stage II and III with stage I (P<0.05).The nuclear overexpression of CDK4 in moderately-poorly differentiated (G2-G3) carcinoma was higher than that of G1 carcinoma (P<0.05). The positive rates of CDK4 in nuclear expression and overexpression in >1/2 muscle invasion cancer were significantly higher than those in ≤1/2 muscle invasion (P<0.05).In 41 cases followed up ,the nuclear positve expression and overexpression rates of CDK4 were significantly higher in 15 cases with recurrence than 26 cases without recurrence (P<0.05).Conclusion: Our results suggest that the aberrant expressions of PTEN and CDK4 are closely related to the occurrence ,development and reccurrence of endometrial carcinoma.