| Backgroud and Purpose:Vascular disease (VD) is induced by cerebrovascular diseases. It is one of the main kinds of senile dementia and the morbidity of VD is high among the elderly. Following the life expectancy rising, the morbidity of cerebrovascular diseases increased rapidly and cerebrovascular diseases have been one of the leading causes of disease death. VD is the main complication of cerebrovascular diseases. In China, VD is a leading cause of senile dementia. China has become aging society, VD will bring about more and more social and economical problems. It's very important and urgent to actively study VD.Neuropeptides are some important substances which have functions of neuronal signal transmission and regulation. They distribute v\ idely in the central nervous system, and participate in many kinds of functional regulation. An Yuhui has isolated some small neuropeptides from bovine brains and found a new peptide:bovine acidic neuropeptide 1 (BANP-1). It is a small molecular peptide and hasn't antigenicity. Animal experiments have proved that it can increase amino acid of brain and affect the contents of cGMP of brain. In this study, the effects of BANP-1 on VD mice were observed by setting up VD mouse model, in which common carotid artery ischemia and reperfusion was used to damage the CA1 subfield of hippocampus on the basis of hyperlipid. The purpose of this research is to accumulate valuable data for researching pathogenesis of VD and to explore new medicine for treatment of VD.Materials and Methods:Eighty-four healthy mice (22~25g) were chosen and randomly devided into 7 groups. Each group had 12 mice. One group was taken as normal control group without any treatment, the other groups were made hyperlipid and performed for common carotid artery ischemia and reperfusion. After the operation, III,IV, V,VI, VII group mice took orally normal saline, Naofukang 0.3g/kg-d, BANP-1 1.5g/kg-d, 0.75g/kg-d, 0.3g/kg-d respectively. Normal control group and model group mice didn't treat. The process had lasted for 15 days, once every day, each time 1ml. The learning and remembering ability was tested by black avoidance test and jumping table test. Then all mice were decapitated, blood was collected and serum was isolated to examine the contents of TG and Teh. The brain was homogenized and centrifugalized to examine Pro, SOD, MDA, NO and AChE. The experimental data were expressed by x + s and analyzed by one-way ANOVA. a =0.05 was the standard of test.Results:1. Comparing with normal control group, BANP-1 groups: Pro increased significantly (P<0.05) ,model and normal saline groups: Pro decreased significantly. The learning and remembering ability of other groups decreased significantly(P<0.05), SOD, AChE decreased significantly (P<0.01),TG, Tch, MDA, NO increased significantly (P<0.01) .2. Comparing with model group, BANP-1 groups: the learning and remembering ability increased signifiantly (P<0.05), Pro> SOD increased significantly (P<0.05,P<0.01) ,NO> MDA decreased significantly(PO.01),TG ^ Teh and AChE had no significant differences(P>0.05). Naofukang group: the learning and remembering ability increased signifiantly (P<0.05), Pro increased significantly (PO.05) , NO decreased significantly (PO.05) , TG, Tch and AChE, SOD, MDA had no significant differences (P>0.05). There was no significant differences in normal saline group (P>0.05).3. Comparing with Naofukang group, BANP-1 groups: the learning ability had no significant difference(P>0.05); 1.5g/kg-d and 0.75g/kg-d groups: the remembering ability increased significantly(PO.05). Pro increased significantly (P<0.01),and all BANP-1 groups: SOD increased significantly (P<0.01 ) , MDA, NO decreased significantly (PO.05) .4. BANP-1 groups mutual comparing, 1.5g/kg-d group: the remembering ability increased significantly ( P<0.05 ) , NO decreased significantly ( P<0.05 ) ; 1 .5g/kg-d and 0.75g/kg-d groups: Pro, SOD increased significantly (P<0.05) , MDA decreasedsignificantly (P<...
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