| Objective:To study the changes of ultrastructure induced by epidural blockade using different concentration of alcohol and phenol glycerin.Methods:20 rabbits were randomly assigned into four groups. Group A was control group,recieved a epidural injection of normal saline.Group B recieved a epidural injection of 75% of alcohol. Group C recieved a epidural injection of dehydrated alcohol and group D 10% of phenol glycerin. The speed of injection was 0.5ml/min.After the puncture and injection,the rabbits were raised under same condition for 7 days. Then a perfused fixation was performed and the spinal nerve and the roots of spinal nerve were taken and observed and shot using a JEM1200EX transmission electro-microscope.Results:Mitochondrials of spinal nerve were denaturalized,microfibril and microtubule of axonemes were vanished,lipofuscin granules were increased,non-myelinated fibrinolysis was occured and myelinated fibre root of spinal nerve were foundeddemyelinated,and hypertrophy of Schwann cells was observed in thegroup of 75% of alcohol. The mitochondrials were decreased in axons ofmyelinated fibre,vacuoles were observed in mitochondrials ofnon-myelinated fibre,and no significant changes was observed in the root of spinal nerve. No significant changes was observed in spinal nerve and root of spinal nerve in 10% of phenol glycerin group. Conclusion:the best dood concentration of drug for NNB is 75% of alcohol,and theefect of epidural NNB is undefined,... |
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