| Objective: Neutropenia is observed in 36%-57% of the neonates delivered to women with pregnancy-induced hypertention (PIH). Observations showed that this kind of neutropenia was generally transient and not due to neonates sepsis. But recently, some studies showed that such neonates might have a higher risk for nosocomial infection in the first few weeks of life. Many scholars tried to find the mechanism under this kind of neutropenia, but the exact mechanism remains unresolved. The aim of our study was to detect the levels of G-CSF, IL-6 and FL in cord blood of neonates bom to mothers with PIH, to explore the relationships among endogenous G-CSF, IL-6 and FL levels, PIH and PIH-related neonate neutropenia, to analysis the hematopoietic growth factors in cord blood more completely, to provide theoretical evidence for implying of cord blood and effective prevention and treatment to PIH-related neonatal neutropenia.Methods: FL, G-CSF and EL-6 levels in the cord blood of 87 neonates (gestational age was 36.5+4.2 weeks, birthweight was 2350+690 grams) were measured by enzyme-linked immunosobent assay(ELISA). hi these neonates, 46 were born to mothers with PIH, 34 were low birth weight infants, 23 were small for their gestational age, and 54 were delivered by cesarean. All of these cases were divided into several groups according to different characters and the data were analyzed with student's t-test, chi-square analysis, spearman rank correlate analysis and logistic regression.Results: (1) The differences of peripheral blood smears between the neonates delivered to mothers with PIH and without PIH. Compared with non-PIH group, infants in PIH group had significantly lower nucleated cells, corrected white blood cells, neutrophils and platelet count (p<0.01); So did lymphocytes between the two groups; While there showed no significant difference of red blood cells, eosinophils, basophils and monocytes between the two groups. (2) Comparisons of neutrophils among groups of different clinical characteristics. The neutrophile count of infants in the preterm group, PIH group, casesrean group and low birth weight group were significantly lower than their correspond control groups respectively (p<0.05); Neutrophiles of infants in the SGA group was also lower than that in non-SGA group, but they showed no statistically signifycant differences. (3) FL levels in different groups. Among the factor groups related to neutrophil count, FL levels in the PIH group was 67.5+9.1pg/ml,significantly higher than that in the non-PIH group (61.4?7.3pg/ml), (t=2.984, p<0.01), but the FL levels showed no significant differences between the other factor groups and their correspond control groups. (4) G-CSF levels in different groups. Among the study groups, G-CSF level in the PIH group (35.2+40.7pg/ml) was higher than that in the non-PIH group (14.9+11 pg/ml), (t=3.706, p<0.01), but there were no statistically obvious differences between the other groups and their control groups. (5) IL-6 levels in different groups. There were no significant differences between the study groups and their corresponding control groups. (6) Correlation between FL, G-CSF, IL-6 and neutrphil count in the PIH group. The levels of FL and G-CSF in cord blood were negatively related to the neutrophile count in the neonates born to mothers with PIH (rs=-0.378 and -0.296 respectively), (p<0.01 and <0.05 respectively); But there was no significant correlation between the level of IL-6 and neutrophil count by spearman correlate analysis. (7) Comparasions of characteristics of infants between neutropenia group and non-neutropenia group. Compared with non-neutropenia group, the infants of neutropenia had younger gestational age (t=4.015, p <0.01), lower birth weight (t=4.080, p<0.01), higher levels of FL (t=3.210, p<0.01) and G-CSF (t=3.978, p<0.01) in their cord blood; And the level of IL-6 was higher, too, but it showed no significant difference (t=1.117, p<0.05). (8)Analysis of the risk factors for PIH-related neutropenia. Logistic regression with neu... |
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