Effect Of Endogenous Human Basic Fibroblast Growth Factor And FGFR1 On Malignancy Of Tumor Cells

Aims:To examine the contribution of endogenous bFGF to the abnormal growth and phenotypic transformation to neoplastic tumors cells.Methods: In the present study ,the influnce of endogenous bFGFon human malignant brain glioma cells tumor SWO-38 cell line,lung carcinoma ASTC-A-1 cell line ,breast cancer BCAP-37 cell line,hepatocarcinoma HepG-2 cell line and laryngocarcinoma Hep-2 cell line growth in vitro was evaluted by down-regulating bFGFexpression using antisense oligonucleotide primers.The characteristic expression of bFGF and FGFR1 was examined by immunohistochemical methods and image analysis techniques.In situ hybridization and image analysis technique was used for bFGF mRNA expression.inaddition,extracellular bFGF was examined by ELISA .MTT spetrophotametry was used to examine the effect of bFGF antisense oligonucleotide primers on survival of the cells.Cloning efficency of the cells in soft agar was assessed after exposed to bFGF antisense oligonucleotide primers.Scanning electron microscopy was used to observe the change of SWO-38 cell surface. ResultrBFGF expression was by the addition of bFGF antisense oligonucleotide primers. The addition of bFGF-specific antisense oligonucleotides to the five types of tumor cells resulted in inhabition(from30% to 51%)in survival of the cells .bFGFantisense oligonucleotides also inhabited the colony formation in soft agar and changed Colony morphology also.These effects were dose-dependent and specific .In addition, FGFR1 expression was found reduced in responds tobFGF-specific antisense primers when we studied SWO-38 cell line and BCAP-37 cell line.There are some changes of SWO-38 cell surface when infected bFGF antisense oligonucleotide primers in electron microscopy.Conclusions:Endogenous bFGF can regulate the phenotypic malignacy in the humor tumor cells.The alterations in bFGF expression may play a role in the development and prosession of human neoplastic tumors.BFGFmay act as auntocrine regulators by interacting with FGFR1 on malignant tumors cells.BFGF ,FGFmRNA or FGFR1 lever in tumor tissue or tumor patientsis a useful giognotic marker. It is hopeful that bFGF antisense oligonucleotide primers may become a new thrapeutic drug for anti-tumor.