| Objective To explore the correlation between incidence of coronary heart disease (CHD) and the changes of molecular markers of coagulation,fibrinolysis and immuno-system in patients with CHD. This may help to diagnosis ,anticoagulation,thrombolysis and prognosis for patients with CHD. Concurrently, these molecular markers may contribute to screen , confirm and treat thrombotic diseases in clinic.Methods Plasma platelet granule membrane protein 140 (GMP-140), von Wille-brand factor (vWF), D-dimer(D-D) and median bound index of serum anticardiolipin antibodies (ACL) were detected by Enzyme Linked Immunosorbent Assay(ELISA) in 100 patients with CHD ,and compared with 42 healthy subjects.Results Plasma GMP-140,vWF , D-D and ACL were significantly elevated in patients with CHD and differed significantly compared with control group(f = 2. 529 -19. 390,P<0. 05). The changes of molecular markers in patients with acute myocardial infarction (AMI) were elevated significantly than those with old myocardial infarction (OMI), unstable agina pectoris (UAP) and stable agina (SA) (t=3. 264-14. 409, F= 2. 472 - 67. 378, P<0. 01). The levels of plasma GMP-140 in patients with UAP were elevated signifi cantly than those with SA(t=3. 236, P<0. 05). The positive rates of IgG-ACL, IgM-ACL and IgA-ACL in patients with CHD were 24. 0%,18. 0%,6. 0% respectively. The positive rates of ACL in patients with AMI were 55. 6%,44. 4% respectively and elevated significantly than those with UAP, SA (x2=4. 547-8. 629,P< 0. 05),and dominated by the types of IgG and IgM. But there is no significant difference between the AMI group and OMI group on positive rates of ACL(^2 =2. 391 - 3. 326, P>0. 05). There were significant correlations between the molecular markers in patients with CHD(r=0. 857 - 0. 301 , P<0. 05 - 0. 01). The levels of vWF and D-D were significantly corre lated with the levels of IgG-ACL and IgM-ACL in patients with AMI (r= 0. 574-0. 351,P<0. 05 - 0. 01). The levels of GMP-140,vWF and D-D were associated with each other closely (r=0. 385 - 0. 425, P<0. 05), while only the levels ofvWF and D-D were correlated with the levels of IgG-ACL in patients with OMI (r = 0. 385-0. 425,P<0. 05). Although the levels of GMP- 140, vWF and D-D were correlated with each other significantly(r=0. 857-0. 434, P<0. 01),they were not correlated with the levels of IgG-ACL.Conclusion There existed an obvious hypercoagulable state in patients with CHD. There were correlations between the changes of molecular markers of prethrombotic state and patients' condition. At the same time, immunual factors contribute to the process and severity of CHD. Combination of quantitative determination of different prethrombotic molecular markers and ACL are valuable for diagnosis of prethrombotic state in CHD , as well as for investigation of the pathophysiological mechanism of this disorder. |
PDF Full Text Request