Influence Of Congenital CWV Infection On The Function Of Type 1 T Helper

Objective To study the influence of peripheral blood virus load of congenital CMV infection to type 1 T helper .Methods Peripheral blood CMV-DNA were detected quantitatively by FQ-PCR in the full term infants in our NICU during 2001.1-2001.12,31 infants with positive result as infection group, 29 non-infected infants with negative result and CMV-IgM as control group. And IL-2,IFNY were detected by ELISA, and the percentage of Th: were detected by single cell dying in all infants. After 4 weeks, CMV-DNA,IL-2,IFNY and Thi were detected again in infection group; IL-2, IFN and Thi were also detected a-gain in control group. Then the results were compared respectively.Results The median of CMV virus load in peripheral blood of infection group was 2. 75 X104copy/ml. The level of IL-2C16. 55 + 2. 87g/L) of infection group was significantly lower than that (24. 73+4. 37/ng/L)of control group (r = 8.6242, P<0.01);The level of IFNy (475. 6 it27. 1 pg/ml)of infection group was significantly lower than that (654. 07+14. 64 pg/ml)of control group(t = 31. 4228, P<0. 01) ;The percentage of Th, (11. 04+2. 51%)of infection group was significantly lower than that (15. 7+2.73%)of control group(f=6. 8887, P<0. 01). The level of IL-2,IFNYand the percentage of Thi correlated negatively with CMV virus load in peripheral blood (r=-0. 7882;-0. 6751; -0. 7082. P<0. 01). CMV was disappear in peripheral blood in 30 infants after 4 weeks, and the level of IL-2(25. 55 + 6. 99fig/L) increased significantly in comparision with the original level(t=6. 8700, P<0. 01); The level of IFNy(647. 65+29.8 pg/ml) increased significantly in comparision with the original level(t = 23. 0149, P<0. 01); And the percentage of Thi (16. 98 + 3. 07%) increased significantly in comparision with the original level(t=7. 0354, P<0. 01).Conclusion The quantity and function of Thi both decline during congenital CMV infection, Thi / Tli2 decrease. There is an abnormality in Th cell polarization . The function of cell immunity mediated by CTL is depressed and the degree relate significantly to virus load in peripheral blood.