The Research Of Ovarian And Cervical Specific Transfer Factor On Anti-tumor In Vitro

Objective: To detect the role of cervical or ovarian STF in the PBMC-mediated cytotoxicity to cervical or ovarian cancer cell lines.Methods: 1. With MTT, the experiment in vitro showed that the effect of PBMC-mediated cytotoxicity on cervical or ovarian cancer cell line was influened by different consistency of cervical or ovarian STF and in different time. 2. With MTT, the experiment in vitro showed that two different experiment steps were taken in the research in order to find out the better one. 3. With MTT, the experiment in vitro showed that the respond cell and target cell were cultured by RPMI 1640+10% fetal bovine serum or RPMI 1640 + 10% AB type person's serum, then observed the effect of the different culture medium. 4. With MTT, the experiment in vitro showed that the different rate of effect PBMC and target cell in the same consistency of STF was designed to detect the best rate of the respond cell and target cell in the experiment. 5. With MTT, the experiment in vitro showed that PBMC which was activated by cervical STF incubated with ovarian cancer cell line and PBMC which was activated by ovarian STF incubated with cervical cancer cell line be compared with PBMC activated by cervical STF incubated with cervical cancer cell line and PBMC activated by ovarian STF incubated with ovarian cancer cell line, then detect if transfer factor is an antigen-specific material. 6. With MTT, the experiment in vitro showed that PBMC be activated by STF and IL-2 to observe the effect of PBMC-mediated cytotoxicity in cervical or ovarian cancer cell line and to find out if the STF has the synergy function with IL-2.Results : 1.The kill activity of PBMC effect cell which was activated by STF in vitro incubation reached the peak after 48 hours . With the increase of the dose of STF, the kill activity of PBMC was enhanced. 2. We could not get the differences when utilizing two different experiment steps and two different culture mediums. 3. We got the best kill activity of PBMC when the rate of PBMC respond cell to target cell was 8:1. 4.Compared with PBMC activated by cervical STF incubated with cervical cancer cell line and PBMC activated by ovarian STF incubated with ovarian or cancer cell line, PBMC activated by cervical STF incubated with ovarian or cancer cell line and PBMC activated by ovarian STF incubated with cervical cancer cell line had lower kill activity of PBMC. 5. PBMC activated by STF and IL-2 enhanced the kill activity of PBMC.Conclusion: 1. Cervical or ovarian STF could enhance the kill activity of PBMC, it is dose dependent and reaches the peak after 48 hours. 2. In the experiment, two different experiment steps and two different culture mediums didn't influence the results. 3. Transfer factor is an antigen-specific material. 4. Utilizing STF with IL-2 in vitro to incubate PBMC has the synergy function.