| Periodontal disease is a disease initiated from periodontal tissues (gingiva, periodontal ligament, alveolar bone and cementum). In Chinese population, the incidence rate of this disease arrives 70%. Peridontal disease often results in loss of teeth, which causes dysmasesia and dyspepsia. Up to now, there are no ideal drugs and treatment. Gingivitis, periodontal pocket formation, alveolar bone resorption and teeth loose are the four clinical symptoms of periodontal disease. Alveolar bone resorption and formation of periodontal pocket are considered to be cardinal of the four symptoms. Alveolar bone resorption is the major reason of dysmasesia and dyspepsia. How to suppress bone resorption and promote bone modeling is the key subject for the therapy of periodontal disease.Chinese medicine herbs are thought as an important source of natural drugs. According to and the principles of Chinese medical science, GSB and DL was selected as the drugs for research.Objective: To examine the effect extracts of GSB and DL on MC3T3-E1 cell and alveolar bone resorption model in SD rat respectively.Methods: GSB^ DL were extracted with distilled water and 95 % ethanol respectively to attain water extract of GSB(w-GSB), ethnol extract of GSB(e-GSB), water extract of DL(w-DL) and ethnol extract of DL(e-DL). MC3T3-E1 cell was taken as the model for experiment in vitro. MTT method and analysis of cell cycle by flow cytometry were used to study the influence of different dosage of the four extracts on MC3T3-E1 cell proliferation. The activity of alkali phosphate of MC3T3-El and the concentration of osteocalcin of cultural medium were used to study the influence of different dosage of the four extracts on MC3T3-E1 cell differentiation. Von kossa calcificational stein were used to study the influence of different dosage of the four extracts on MC3T3-E1 cell calcification.Alveolar resorption model was established by injection lipopolysaccharide of E. coli O111:B4 to the mesial gingiva of the first molar on the left mandible of SD rat. The injection was repeated every 2d during 8d. The SD rats were treated with different dosage of the four extracts respectively. On the basis of histological observation and tartrate resistant acid phosphatase stein, the effects of different dosage of drug extraction were evalueted.Results: The results of MTT showed some dosages of the four extracts promoted MC3T3-E1 cell to proliferation, differentiate and calcificate in intro. 1X10?mg/L and 1 X 1(T2 mg/L w-GSB and 1X10?mg/L w-DL raised the capability of MC3T3-E1 to proliferate ( P < 0.05 ), 1 X 10?mg/L ^ 1 X 10"2mg/L e-DL significantly raised the capability of MC3T3-E1 to proliferate ( P < 0.001 ).The results of analysis of cell cycle demonstrated that some dosage of the four extracts were able to shorten MC3T3-El's cell cycle, exceeding each times-phase. The percentage of cell of S phase increased, and that of cell of G, phase decreased accordingly. However, the effect of 1 X 102mg/L w-GSB and 1 X 10?mg/L e-GSB on MC3T3-E1 cell was not significant.1 X 102 mg/L > 1 X 10?mg/L e-GSB raised ALP activity of MC3T3-E1 cell( P < 0.05 ). 1 X 10'2 mg/L w-DL and 1 X 10?mg/L e-DL increased the capability of MC3T3-E1 cell to synthesis osteocalcin during 1st - 3th d since adding the extracts. 1X 102mg/L e-GSB and e-DL during 7th - 9th d since adding the extracts, 1 X 102mg/L w-DL during 10th ?12th d since adding the extracts increased significantly the capability of MC3T3-E1 ceil to synthesis osteocalcin( P < 0.001 ).1X10?mg/L w-GSB, 1 X 10'2 mg/L w-DL, 1 X 10'2 mg/L e-GSB and 1 X 10~2mg/L e-DL increased cell's capability of calcification( P < 0.05 ). IX 10?mg/L w-DL increased the capability significantly ( P < 0.001 ).The results of every extract on the SD rat models of alveolar bone resorption indicated each dosage of the four extracts mentioned above inhibited alveolar bone resorption and improved their bone modeling. On 10th day, the group of w-GSB 1.5 g, the group of w-DL 3 g and 1 g, the... |
PDF Full Text Request