The Protectlve Effects Of Polypeptides From Chlamys Farreri On Hela Cells And Hairless Mice Damaged By Ultraviolet B

AIM In this paper we studied the protective effects of the polypeptides was isolated from Chlamys farreri (PCF, Mr: 800-1000) using enzyme engineering technique on Hela epithelia cells and hairless mice damaged by ultraviolet B. MATHODS We established the oxidative damage models of Hela epithelia cells and hairless mice irradiated by ultraviolet B to study ( cells: UVB 7. 15x lOJ/cm2; mice: UVB 5.15x I0'2]/cm x 30 day). The Hela cells were randomly divided into six groups: control group,; UVB radiated groups ( RPMI-1640 model, 0.5% PCF, 1% PCF, 2% PCF, 1%VitC ). The enzymic activities of Hela cells were tested by MTT methods; The rates of apoptosis and mortalities of Hela epithelia cells and the free calcium in those cells were tested using flow cytometry ( FCM ) ;The concentration of GSH-Px, SOD, CAT, and MDA in the supernates of cells were determined by biochemical and respectively. The mice were randomly divided into five groups: control, UVB radiated groups ( Water model, 5%PCF, 20%PCF, 10%VitC).The numbers of fibroblasts and the thickness of. epidermis were counted under microscope, and the ultrastructures of skin were observed by electromicroscope. The expression of Bcl~2 protein and NOS were examined byimmunochemical methods. RESULTS The results indicated that PCF could enhance the activities of GSH-Px, SOD, CAT and decrese the contents of MDA in Hela cells, the concentration of cellular free calcium in the cells , the rates of apoptosis and mortalities of the cells . The thickness of epidermis and the amounts of f ibroblasts in skin of mice were increased by PCF. The large dilatant vacuolations and few rough endoplasm reticulum in fibroblasts and epidermic cells of mice in UVB model group were observed under electromicroscope. But the ultrastructure of epidermis and fibroblasts in the mice of PCF groups remained normal; and the rough endoplasm reticulum were much more in the fibroblasts of the mice in PCF group than that in UVB group . The PCF could decrease the amounts of MDA and increase the contents of SOD and T-AOC in the skin. It also could enhence the expression of Bcl-2 protein and inhibit the activity of NOS (P<0. 05). CONCLUSION The PCF has the protective effects on the hairless mice irradiated by ultraviolet B. The mechanism of this effects of PCR may be mediated by eliminating the free radicals, by increasing the activities of antioxidative enzymes to inhibit the oxidative damages of UVB and by enhencing the expresses of Bcl-2 protein and decreasing the NOS to reduce the occurrence of apoptosis in the cells of skin...