Exoression Of VEGF,NOS2 And NOS3 In Giant Cell Tumor Of Bone And Their Relation To Angiogensis

Objective To analyse vascular endothelial growth factor (VEGF), inducible nitric oxid synthase (iNOS, NOS2) and endothelial nitric oxide synthase(eNOS,NOS3) expression in giant cell tumor of bone(GCT) and their relation to angiogenesis.Summary of background data VEGF and NOS are two most important factors relating to angiogenesis, both VEGF and NOS were found high expression rate in many kinds of tumors, such as lung cancer, gastric cancer, brain cancer, hepatocellular carcinoma and so on, but their expressions in giant cell tumor of bone and their relation to angiogenesis had not been reported yet.Methods Tissue section from 49 cases of GCT patients were examined immunohistochemically for protein expression of VEGF N NOS2 and NOS3. Microvessal density (MVD) was counted by endotheilial cells immunostained by anti-CD34 antibody.Then using t' tes, analysis of variance and x 2 test to analyse the results.Results Positive VEGF , NOS2 and NOS3 immunos tain ing was dete in 61. 2% , 57. 1% and 71. 4% of GCT respectively. The MVD significantly correlated with pathological grade, III > II , III >I. MVD was 37. 7 ± 10. 5/HP, 37. 9 ± 10. 9/HP and 35. 4 ± 11. 7/HP in GCT positive for VEGF> NOS2 and NOS3 while it was 24.5 ± 6. 8/HP> 25. 2 ± 7. I/HP and 25. 6?. 8/HP in GCT negative for VEGF > NOS2 and NOS3 respectively (P<0. 01). The expression of NOS2 and VEGF in GCT was significantly correlated with each other (p<0.05), but the correlation between NOS3 expression and VEGF in GCT was not observed. (p>0.05)Conclusion The MVD in GCT was correlated with the histopathological grading. The expression of VEGF> NOS2 and NOS3 favours angiogenesis of GCT, and The expression of NOS2 and VEGF in GCT was significantly correlated with each other.