The Effect Of Liver-regulating And Turbid-removing Prescription On Experimental Hyperlipemia And Atherosclerosis

Objective: To observe the effect of liver-regulating andturbid-removing prescription on lipid in blood and pathological changes of liver and aorta of experimental hyperlipemia model of rat and experimental atherosclerotic model of rabbit, study the pathogenesis of atherosclerosis (AS) and the mechanism of Chinese herbs on regulating lipid metabolism and preventing or curing AS.Method:1. Hyperlipemia model of rat was established by having been fed heavy dosage of cholesterol for two months. The Chinese herbs and the controlled drug were given according to grouping in the course of feeding. After experiment ended, put the object to death and sampled the liver and blood.2. Atherosclerotic model of rabbit was established by having been fed heavy dosage of cholesterol for three months. The Chinese herbs and the controlled drug were given according to grouping in the course of feeding. After experiment ended, put the object to death and sampled the aorta and blood.3. Aortic smooth muscle cells (SMC) were cultured in vitro and done in groups with different culture medium-the serum containing high concentration of cholesterol and the sterilized Chinese herbs extract. In order to study the regulative action of Chinese herbs on cell proliferation, indices in growth factor, oncogene and messengersystem were assayed by using the immunohistochemical method.4. The hepatocytes of rat were cultured in vitro in different groups by adding the sterilized Chinese herbs extract into the culture medium and the serum containing high concentration of cholesteral. 24 hours later, the cells were fixed with paraformaldehyde containing DEPC and the analysis was made on the mRNA expression of low density lipoprotein (LDL) receptor with hybridization in situ.5. The mice had been fed by heavy dosage of cholesterol for two weeks, and the Chinese herbs were given in the course of feeding. After experiment ended, put the object to death and sampled the blood and collected the macrophages (MO) in the abdominal cavity. The content of oxidized low density lipoprotein (OX-LDL) in blood was assayed with enzyme linked immunosorbent assay (ELISA). The total RNA of MO was extracted by trizol method, and the mRNA expression was analyzed upon macrophage colony-stimulating factor (M-CSF) by means of reverse transcriptase polymerase chain reaction (RT-PCR).Results:1. The contents of cholesterol(Ch), triglyceride(TG), LDL in blood of Chinese herbs group decreased more significantly than those of the model group and the content of high density lipoprotein (HDL) in blood of Chinese herbs group increased more significantly than that of the model group. The pathological changes of liver showed that the Chinese herbs could obviously reduce fatty degeneration of hepatocyte.2. In the experimental atherosclerotic model of rabbit, the Chinese herbs could effectively inhibit the proliferation of cells and prevent the formation of AS plaque.3. The Chinese herbs could inhibit proliferation of SMC cultured in vitro by inducing the expression of nitric oxide synthase (NOS) and producing nitric oxide (NO). It was also observed that the platelet-derived growth factor-A (PDGF-A) and oncogene c-myc expression of SMC in the Chinese herbs group were significantly inhibited.4. The results of hybridization in situ showed that the serum containing high concentration of cholesteral might cause the mRNA expression of LDL receptor in the hepatocyte membrane to decrease. Compared with the controlled and model group, the mRNA expression of LDL receptor in the Chinese herbs group could be obviously strenghthened.5. In compassion with the controlled group, the contents of OX-LDL in blood of the model group rised. RT-PCR result showed that the mRNA expression of M-CSF of the model group was highly more reinforced than that of the controlled group. While in the Chinese herbs group, there was only a slight oxidatively modified reaction and moderate enhancement of mRNA expression of M-CSF.Conclusion: The Liver-regulat...