Modeling Of Rabbit Renal VX2 Tumor With Ultrasound-guided Implantation Of Tissue Mass And Ultrasonographic Observations On The Growth Of Rabbit Renal VX2 Tumor

Purpose: To compare the modeling of rabbit renal VX2 tumor with the intraoperative mass embedding to with ultrasound-guided implantation of constitution mass. To explore the regularity of the ultrasonographic dynamic changes such as the size, echo-intensity and edge echo-pattern of renal tumor during its growth. To offer the animal experimental proves of the preclinical phase for the early diagnosis and treatment of renal carcinoma.Materials and methods: Sixty-six New Zealand rabbits were randomly divided into team of ultrasound-guided implantation of tissue mass (58) and team of intraoperative mass embedding (8). The tumor stud was VX2 squamous carcinoma. The anesthesia drug was 846 mixtures. The tissue mass of 1mm3 size was produced by separation and shearing. Then added in saline and got suspension. Fifty-eight rabbit left renal cortex were implanted with 0.1ml VX2 tumor constitution mass suspension guided by ultrasonography. Eight rabbit left renal cortex were embedded with tissue mass of 1mm3 size after operation exposure of left kidney. Acuson Sequoia 512 computer sonography with video-density quantitative determination software was employed. The ultrasonographic observation was performed on the 7, 10, 14, 17 and 21 day after implantation. The survivals were put to death and necropsy. The section preparations were stained with HE method.Results The implant success rate of ultrasound-guided was 96.55% (56 / 58) , while that of the intraoperative mass embedding was 50% (4/8) . There was significant statistical difference between the data. The abdominal wall implantationrate of ultrasonography-guided was 3.4% (3/56) , while that of the intraoperative mass embedding was 25%. There was no significant statistical difference between the data. The average sizes of the tumor in each observing time were 0.59?0.19cm, 0.86 + 0.260TK 1.11 ?.36crru 1.39?.49cm and 1.78?.71cm respectively, which was in positive correlation with the time after the implantation. The echo-intensity of the tumor can be divided into three patterns such as hyperecho, isoecho and hypoecho. The edge echo-patterns of the tumor could be divided into four patterns such as "Halo Sign", "Bull Eye Sign" and "Lighted Rim Sign". The observed rate of abnormal vessels in the tumor increased as the implantation time elongated and the tumor enlarged. The observed time of arterial blood was earlier than that of venous blood. The effective factors of histopathology mostly were necrosis, fiber and sinusoid.Conclusion: The histological manifestation of the modeling of rabbit renal VX2 tumor with these two methods was identical to that of stud. The modeling of rabbit renal VX2 tumor with ultrasound-guided implantation of tissue mass was prior to that of the intraoperative mass embedding in implant success rate, abdominal wall implantation rate and experimental cost. The ultrasonographic signs such as the size, echo-intensity, edge echo-pattern and blood flow changes of renal VX2 tumor during its growth were coincident to clinical renal tumor. The gradual growth and gradual behavior of ultrasonography were essentially coincident to the definitive characteristics of tumor invasion. This animal model can be used to observe the malignant biological behaviors. The sizes of most tumors were less than 3 cm, therefore the dynamic observation of the ultrasonographic routine parameters is useful to the diagnosis and identification of the clinical little kidney tumor.