| 20 rabbits were divided into I - V five groups (4 rabbits per group) randomly, the I group was fed normally as negative control group; the II group as positive group. The II-V four groups were injected lead acetate solution into their abdomen at a dosage of 1.57mg/kg every 48 hours for 20 days. Simultaneously, except the II group, the IIL iV N V three groups were fed every day with garlic oil at a dosage of 70mg/kg, 140mg/kg and 420mg/kg respectively, continuously observing for 40 days. Urine and feces of each group rabbits were collected ever}' 4 days. The content or concentration lead. Zinc, Ferrum and Copper in urine, feces, organs, tissue and blood of five groups rabbits was determined by AAS after the 40 days. The result showed that the lead content in urine, feces. brain, liver, kidney, bone and lead concentration in blood of the II group rabbits were significantly higher than that of the I group; the lead content in mine and feces of the III- Y groups was significantly higher than that of the II group, lead content in feces was higher than that in urine, and the more dosage of garlic oil . the more lead excretion of III-V groups: lead content in organs and tissue of the III- V group rabbits was significantly lower than that of the II group; lead concentration in blood of the III- V groups retained the level of the I group, and3the more dosage of garlic oil , the lower that of III-V groups. The content of Zinc, Ferrum and Copper in urine of the II group was significantly higher than that of the I and III- V group rabbits; the content of Zinc, Ferrum and Copper in urine of the III- V groups rabbits had no significant difference compared with that of the I group, and the more dosage garlic oil, the lower that of III-V groups. Compared with the I group, the concentration of Zinc, Ferrum in blood of the II group rabbits failed, and the Copper concentration in blood of the II group raised. The concentration of Zinc, Ferrum, Copper in blood of the IV-V groups had no significant difference compared with that of the I group; the concentration of Zinc and Ferrum in blood of the III-V groups was higher than that of the II group, the concentration of Copper in blood was lower than that of the II group. The content of Zinc, Ferrum, Copper in organs of the II group was significantly lower than that of the I group; except the content of Ferrum in liver ,the content of Zinc, Ferrum, Copper in organs of the III- V groups was higher than that of the I group, and the more dosage garlic oil, the more that of III-V groups. The Zinc, Ferrum and Copper contents in organs and Zinc, Ferrum concentration in blood of II - V groups was negatively related with lead contents or concentration of the corresponding groups, and Copper concentration in blood of II - V groups was positively related with lead concentration in blood of the corresponding groups. The experiment indicated firstly that the garlic oil could advance the excretion of the lead and reduce the lead accumulation level in tissue and organs of rabbits, especially protecting brain from poisoning. Garlic oil had no significant side effect compared with the traditional antidote of lead poisoning, and not lack of trace elements as Zinc, Ferrum and Copper, etc. Simultaneously, it was found that the content of Zinc, Ferrum and copper had oppositional effect on lead content. The study gave some valuable information for study of active substances and mechanism of detoxification on lead poisoning of garlic or its products and new animal model. |
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