Study On The Intra－ocular Bio－compatibility Of PSBM

Objective　To study the bio-compatibility and biodegration of PSBM ( Polysaccharide Biomembrane ) .　 Methods PSBM, which was made into grafts sized as 2 ( 3mm2 and sterilized in autoclave, was implanted on the surface of rabbit's iris through limbus incision ( 3mm long ) , while the control eyes were only incised and sutured at limbus for comparison. The rabbits' intraocular pressure was measured and recorded regularly, the changes of PSBM grafts were observed with slit-lamp microscope. During the experiment rabbits' eyeballs were excised at the 15th , 25th , and the 55th day respectively to observe the bioactivity of corneal endothelium, the morphological and histological changes of lens epithelium, local iris and retina under light microscope. Moreover, the enzyme activities of lactate dehydrogenase (LDH) , succinate dehydrogenase (SDH) , acid phosphatase (ACP) and alkaline phosphatase (ALP) in lens epithelium and retina were measured with histochemical methods, and the experimental data was analyzed with statistics software-SPSS 8.0. 　 Results　 ① PSBM grafts could be biodegraded gradually and absorbed entirely in animal model's anterior chamber, and the average span of absorption was 25 days. ② The intraocular implantation of PSBM grafts had no definite effects on the bioactivity of corneal endothelium and the morphological structure of lens epithelium and retina, though some slight fibrosis could be observed at local iris. ③ The difference of the 4 enzymes' (LDH, SDH, ACP and ALP) activities between tested and control eyes was of no statistical significance. Neither was that between the tested rabbits' eyes of the 3 experimental groups. 　 Conclusion 　① PSBM has good intraocular biocompatibility and biodegration , which reflects that PSBM would be a promising biomedical material in ophthalmology field. ② PSBM has no definite effects on the 4 kinds of enzyme metabolic activities of lens epithelium and retina , bioactivity of corneal endothelium, morphological structures of corneal endothelium , lens epithelium and retina after being implanted into anterior chamber.