Epidemiologic Study Of P53 Abnormalities And GSTM1 Genotypes In Lung Cancer Patients And Their Family Members

Epidemiologic Study of P53 Abnormalities and GSTM1 Genotypes in Lung Cancer patients and their family membersABSTRACTPURPOSES:To detect the P53 abnormalities and GSTM1 genotypes in lung cancer patients and their family members, investigate the significance of various specirnens(surgical material, sputum and serum) in P53 abnormalities detection, and evaluate the value of P53 and GSTM1 in lung cancer early prediction and monitoring of population at high risk.METHODS:62 cases of lung cancer and 63 their family members, 30 non~ cancer patients as first control group, 47 healthy persons as second control, were detected following indexes by corresponding methods:1P53 gene mutation was detected in surgical and sputum specimens byPCR-SSCP2p53 overexpression was measured in surgical specimens by immunohistochemistry method3p53 overexpression was examined in serum specimens by ELISA4GSTM1 genotype was tested by double PCRRESULTS:1The rates of P53 gene mutation and protein overexpression in lung cancer patients were significantly higher than those in control groups.2P53 gene mutation in sputum was in close relation to that in surgical material.3p53 protein overexpression in serum was related to the one in surgical material.4(3STM1 null genotype occurred more often in lung cancer patients, and was related to P53 gene mutation.CONCLUSIONS:1The tumorigenesis of lung cancer is in close relation to P53 gene mutation and protein overexpression.2Co-analyzing the results of P53 gene mutation in sputum and p53 protein overexpression in serum has further epdemiologic significance in screening and monitoring of high risk population of lung cancer.3Susceptibility of lung cancer increases in individuals with GSTM1 null genotype.4P53 gene mutation is related to GSTM1nu11 genotype.