| BACKGROUND AND AIMS: It has been proved that all malignant tumors are nearly correlated with chromosomal alterations. Investigations on chromosornal alterations of esophageal cancer would contribute to illuminate the pathogenesis of esophageal cancer. H5E973 was a HPVI8 E6E 7-immortalized human embryonic esophageal- epithelial cell line with the inclination towards malignant transformation,and part of its passage 61 cells has been malignantly ti-ansformcd.H5E973 and tumor cell line LTPA established by malignant transformation of H5E973 induced by TPA provided an ideal model for in vitro studying esophageal carcinogenesis. Investigation on chromosomal variation regularity of esophageal epithelial cells during immortalization and malignant transformation was performed on the two cell lines, so as to provide valuable information for further study of molecular cytogenetic abnormalities in esophageal cancer. METHORDS: H5E973 and LTPA cells were cultured and passaged, and metaphase spreads of the two cell lines were prepared and stained by G-banding. The present study analyzed the variation of chromosomal modal number among H5E973 passage 26,43,89 and LTPA passage 63, and performed the G-banding karyotype analysis on H5E973 passage 26,89 and LTPA passage 63. Moreover, irnmunohi stochemistry and image analysis techniques were used to detect the expression of I-JPV 18 E6 and E7 protein in I-15E973 passage 20,85 and LTPA passage 74. RESULTS: Modal number of H5E973 passage 26,43,89 and LTPA passage63 were 56-62,58-63,61 -64,59-64,respectively. Hyperdiploidy and hypotriploidy were dominant in the two cell lines, and in H5E973, the modal number moved right slowly and hypotriploidy increased with the increase of passage.The numerical increases of chromosomes 12,14,17,20 and loss of 6 chromosome Y often occurred in H5E973 passage 26,89 and LTPA passage 63. The present study mainly found 18 identified chromosomal structural alterations relating to chromosomes 1,2,4,5,6,7,8,9,10,13,14,19 and 21 in H5E973 and LTPA cells. Among these alterations, the frequent occurrence of der(4),t(4;?)(q2 1;?), der(5),t(5 ;?)(p 15;?), der( 1 0),t( 1 0;?)(q23), der( 13),t(1 3; 14)(l3qter 梸 cen 梸 l4qter), der(14),t(14;21)(q24;ql 1), der(19),t(19; 1)(q12;p 13), 2lp? d.er(?),t(?; 1)(?:: 1q11 梸 1 qter), der(?),(?;i)Q?::lql I 梸 1q43) were the common characteristic of H5E973 passage 26,89 and LTPA passage 63. del(7)(q3 1) was common in H5E973 passage 26 and 89,but wasn抰 observed at the 6 kaiyotyoes analyzed in LTPA passage 63. der(9),t(9;9) (p24;ql 1) and der(4),t(4;2)(q21 ;q12)were mainly present in I-15E973 passage 89, der(9),t(9 ;9)(9qter梸 cen梸 9qter) was mainly present in LTPA passage 63, der(13),t(13;13)(l3qter 梸 cen 梸 l3qter), del(6)(q2 1?), i(7p), exhibited mainly in I-15E973 passage 89 and LTPA passage 63,while the 6 alterations were less found in H5E973 passage 26. There were the expressions of HPV 18 E6 and E7protein in all of 1-15E973 passage 20,85 and LTPA passage 74,but the expression intensity were different. The expressions of E6 and E7 proteins were the strongest in H5E973 passage 85, but were relatively weak in H5E973 passage 20, and the expression of E7 protein were stronger than that of E6 protein in every passage. CONCLUSIONS: 1. Hyperdiploidy and hypotriploidy were dominant in the... |
PDF Full Text Request