Influence Of Glycine On LBP/CD14 MRNA Expression Induced By LPS

Objective: Lipopolysaccharide(LPS) is a major constituent of endotoxin in the outer membrane of all gram-negative bacteria, its molecule is big and has high bioactivity. Lipopolysaccharide-binding protein(LBP)/cluster of differentiation 14(CD14) systems play an important role in recognizing and regulating LPS action, Our previous studies have shown that glycine(GLY) can effectively antagonize endotoxin action. In the fUrther research, we have found that glycine can alter the structure of LPS, lower the rate of combination of LPS with monocytes, resulting in monocytes!macrophages to produce and release fewer bioactive factors induced by LPS, such as TNF-x, IL-I, et at. This experiment is to further study the effect of glycine on the LBP/CD4 mRNA expression induced by LPS in vivo, and provide theory bases for anti-LPS drug and therapeutic approaches to endotoxin disease. Methods: The level of LBP mRNA expression in the hepatic tissue of rats was examined using RT-PCR, and the effect of glycine on LBP mRNA expression in the hepatic tissue of rats induced by LPS was investigated. The level of CD14 mRNA expression in mouse peritoneal macrophages induced by LPS was detected with in situ hybridization and the effect of glycine on CD14 mRNA expression of LPS-treated mouse peritoneal macrophage was also studied Results: The level of LBP mRNA expression in the hepatic tissue of rats in the LPS group was significantly higher than that of the control group(P