| Purpose: To examine and evaluate the ability of spontanousregeneration following various extent of incomplete injury in aduitrats.Metbods: The quantitive and reduplicated varius extent incompleteinjuried model of adult rats optic nerve was established using differentwounding time with constant wounding force produced by acrossaction forceps at 2mm behind eyeball. GAP-43 (growth associatedprotein-43) and its mRNA erpressions wer detected withimmunhistochemistry (IHC) and reverse transcription polymerasechain reaction (RT-PCR); The site and processes of spontaneousregeneration wer observed by means of anterograde tracing offast-DiI, high affinity fluorescent dye to the neuron and axon, Whichinjected into the vitreous cavity, and the changes of ultrastructure wasviewed by eletron microscope at different recovery time pointsfollowing various extent incomplete injury. All data were analyzed byANOVA.Results: The model can be divided into three groups of mild, moderate,and severe extent of injury, equal to the ratio of across area of 70%,440% and l0% resPecthely GAP43 and its mRNA exPressiOn levelsrevealed tha there was negathe resPonse at the distal area cond tothe theng posithe resPonse at the PrOxha in earfor periOd after..mJmp Until hafmoutIL One mouth lateL the increased GAP-43 and itsInRNA MssiOn levels became mor and mor higher and reachedthe cller at secOnd month POstinny Then it decreased ~llyThe anterograd labeling flUorscent intensity of fastDif was tighlyrelated tO the site and PrOcesses of axon regeneratioll, and qultecoincident to the results of GAP43 and it InRNA exPressbo levels.The results of electrOn microscOPe demonstrated tha ther wer a partof non-myelinated axons tha can sPOntanously regenerate into distalarea ofinjny indeed.Conclusion: Spontanous repeiOn of aduit rats OPti nerve can bedetected and thentified fOllowing incomPlete injny and the extent ofrewi ability is cormlated wun the exten of injmp...
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