Mapping Of The Mutant Gene Of Kunming Sparse Mouse And Studying Its Pathology

Name: Lu Yun (Microbial and Biochemical Pharmacy) Tutor: Bai Xiufeng Kong Xiangyin Alopecia is a common illness in humans and the incidence of this disease is from 20% to 24%. It has many phenotypes such as androgenetic alopecia, alopecia areata, congenital alopecia, telogen effiuvium and so on. Alopecia can result from inherent and environmental causes, such as food, pressure and so on. However, we have a poor knowledge about the genes that cause alopecia in humans. As mammalian models, hairless and hairloss mutant mice play a key role in searching new genes and finding their function. Hair growth abnormalities can result from a variety of mutations in mice. Many correlative genes have been mapped in spontaneously or derivationally mutant mice. As the development of the Human Genome Project (HGP), many new ways of genomic mapping have been produced. Genomic screen by microsatellite DNA Markers and AFLP are more useful than other techniques. In this article we used the way of genetic screen to map the mutant gene and it was mapped to mouse Chr 19. We also founded a way mapping by AFLP and compared the two ways. And we think genomic screen by microsatellite DNA Markers is a quicker and more effective way than mapping through AFLP. 3 In order to map the mutant gene, we used two backcrosses. Cross 1 was (B1OSnxKM) F1xKM and Cross 2 was (DBAxKM) F1XKM. Then by linkage analysis, the mutation was mapped to the distal half of Chr 19. In total 158 microsatellite DNA Markers were used and the mutation was already localized to a relative 6cM chromosome region bounded by two flanking markers, Dl 9mitl 0 and Dl 9mit9O. There are many potential candidate genes such as Cbuk, FgfS, Scdl and Sgl in this region. Thus we offer a base for refining this map and searching the function gene of Kunming sparse-hair mouse. In this paper we also did the histological examination of km/km mouse and the result showed that our autosomal recessive mutant mice also have abnormal sebaceous glands and hair follicles. In the mutations affecting the development of hair in mice several also show abnormal sebaceous-gland development. This makes our mutant mice be a good mammalian model for the studies of the role of skin appendages, such as sebaceous gland and hair follicle, in the hair growth and the interaction between them.