Vascular Endothelial Growth Factor And P53 Expression In Brain Astrocytoma And Their Relation To Angiogenesis

Vascular Endothelial Growth Factor and p53 Expression in Brain Astrocytoma and their Relation to Angiogenesis Objective In this study, we sought to determine a correlation between p53 protein detection, VEGF expression together with intratumoral microvessel density (IMVD) in astrocytoma, and investigated their clinical pathological importance. Methods Surgical specimens from 60 patients with brain astrocytoma were stained immunohistochemically for p53, VEGF and factor VIII. Results Positive p53 protein accumulation and VEGF expression was found in 45% and 65% of tumors, respectively. p53 and VEGF staining status was coincided in 70% tumors, and a significant correlation was found between p53 and VEGF status. Furthermore, the status of p53 and VEGF expression contributed to significant diffrence in IMVD counting as follows: the IMVD was significantly higher in p53-positive or VEGF-positive tumors than in negative tumors, and IMVD in both p53- and VEGF-positive tumors was significantly higher than in the other subgroups. p53, VEGF expression and IMVD were significantly correlated with histopathological grade in patients with astrocytoma, respectively. Conclusions (1) mtp53 protein detection , VEGF expression and IMVD can be considered as an indicator of malignancy potential in patients with brain astrocytom (2) Taking both parenchyma and stroma of astrcytoma into comprehensive consideration, the detection of mtp53 and VEGF simultaneously as well as the determination of IMVD may present more reference and biological information in pathological diagnosis, evaluation of prognosis and clinic treatment; (3) Both mtp53 and VEGF may play crucial role in tumor neovascularization, and conbinating analysis of the status of p53 and VEGF expression may be helpful to understanding of mechanism of angiogenesis of tumor. (4) The results also suggested that there may exist a functional pathway of p53 -VEGF regulating tumor angiogenesis, therefore, providing new strategies and acdemical basis for anti- angiogenic therapies in tumor.