| Helicobacter pylon (H.pylori) has been attracted more attention since it was separated from gastric mucosa and cultured successfully by Marshall and Warren in 1982. 1-Lpyloni is a most important etiological factor of chronic gastnitis and close association with peptic ulcer. It has also near relationship between l-Lpylori and gastric carcinoma (or Mucosa Association Lymphoid Tissue Lymphoma). In 1994, IARC listed H.pylori as Grade I Carcinogen. hi recently, it has been indicated that coronary heart disease, acute myocardial infarction, child growth retardation and Guillain-Barre syndrome is also close association with infection of H.pyloni. H.pylori is a worldwide epidemic bacteria. There are aboute half peoples infected H.pylori. H.pyloni can exist in humam body for several decades, even as long as human life. It is very important to research -4--- 2001 ~ vaccine to prophylaxis the infection of H.pylori. On the early stage, the antigen is the ultrasound fragmenting components of outer membrane. The ultrasound fragmenting antigen contain more components, so there exists crossing response and adverse effect. Later, the vaccine antigens is experienced for lipopolysaccharide, adhesin, heat shock protein, urease, VacA, CagA and catalase. Each antigen has its virtues and shortcomings. I-l.pylori are located in the stomach mucosa and the component of outer membrane can be absorbed into the?circulation and invoke the immune response. So there are component of outer membrane in serum. Little studies have been carried out for detecting H.pylori antigen in serum. In recent, some literatures indicate that there is presenting 1-I.pylori soluble antigen and antigen-antibody complex in serum from patients with H.pylori infection. Previous antigen for vaccinating animal is H.pylori general component in outer membrane,it is crude and has a lower specificity. Now more and more scholars are focused on the urease antigen. H.pylori is featured for high activity urease, The active part of urease is locating outer membrane. Being vaccinated antigen, the recombination urease subunit A and B can protect 60%-80% mouse from infecting H.felis, but subunit B is better than subunit A. It hints that subunit B is a highly specific antigen. But there are also more difficulties in the research of urease antigen, it is marked for the strain diversity of H.pylori. So we synthesis the UreB core polypeptide according to H.pylori ureB conserved sequence. We Vaccinated caprine with UreB core polypeptide for inducing polyclonal IgG antibody. An enzyme-immunoassay is created to detect H.pylori free UreB and its immune complexes in serum. We want -5? 2001 ~ to verificate if there exists UreB in serum from patients with H.pylori infection, and discuss the relationship among H.pylori free UreB and its immune complexes in serum. Thereby we are to provide some theoretic for UreB core peptide as vaccine antigen. All patients are diagnosed by H.pylori IgG, Rapid urease test, MB Stain and Urea Breath test ( any two positive of IgG, Rapid urease test or Urea Breath test and MB Stain is diagnosed as positive). There are 61 patients infected H.pylori among 142 clected patients. In this study, we find the positive rate of fr... |
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